Skip to Content
Merck
CN

Key Documents

View All Documentation

76704

modified Tryptic Soy Broth- Dehydrated Culture Media

NutriSelect® Plus, novobiocin 0.02 g/L, according to ISO 16654:2001, powder, selective for enterovirulent E. coli (EEC)

Synonym(s):

Modified Tryptic Soy Broth with Novobiocin, Modified Tryptic Soy Broth; EHEC enrichment broth, mTSB Broth with Novobiocin

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
NA.85
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Plus
Agency:
according to ISO 16654:2001
Form:
powder
Suitability:
selective for Escherichia coli, selective for coliforms, selective for enterovirulent E. coli (EEC)
Application(s):
agriculture
food and beverages
microbiology
pathogen testing
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

agency

according to ISO 16654:2001

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited, expiry date on the label

composition

bile salts No. 3, 1.5 g/L , di-potassium hydrogen phosphate, 4.0 g/L , D(+)-glucose, 2.5 g/L , novobiocin, 0.02 g/L , peptone from casein, 17.0 g/L , peptone from soymeal, 3.0 g/L , sodium chloride, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

packaging

pkg of -500 g

storage condition

(Dry place, tightly sealed containers)

technique(s)

microbiological culture: suitable

color

faint brown

final pH

7.3±0.2 (25 °C)

application(s)

agriculture
food and beverages
microbiology
pathogen testing

shipped in

wet ice

storage temp.

2-8°C

suitability

selective for Escherichia coli, selective for coliforms, selective for enterovirulent E. coli (EEC)

General description

mTSB Broth with Novobiocin is suitable for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods intended for human and animal consumption. The peptone provides amino acids and other complex nitrogenous substances. Glucose is a fermentable carbohydrate. Sodium chloride delivers osmotic balance. Bile salts inhibit the growth of Gram-positive accompanying bacterial flora. Di-potassium hydrogen phosphate is the buffering agent to stabilize the pH.mTSB is made selective for E. coli O157:H7 by adding bile salts in the media along with Novobiocin supplementation.

Application

mTSB Broth with Novobiocin is recommended for the selective enrichment of enterohemorrhagic E. coli (EHEC) in foods, especially meat and poultry products.The medium meets the requirements of the DIN Norm 10167 for the detection of E. coli serotype O157:H7 in foods. It is also recommended by the FDA-BAM as a method for the isolation of enterohemorrhagic E. coli (EHEC).

Preparation Note

Suspend 33 g in 1 litre of distilled water, autoclave at 121°C for 15 minutes.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Still not finding the right product?

Explore all of our products under modified Tryptic Soy Broth- Dehydrated Culture Media

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动物来源培养基

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCN9563
BCCN0731
BCCM6018
BCCM4072
BCCK0805

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Isolation and molecular characterization of Salmonella enterica,
Escherichia coli O157:H7 and Shigella spp. from meat and dairy products
in Egypt
Ashraf M. Ahmed and Tadashi Shimamoto
International Journal of Food Microbiology, 57-62 (2014)
Optimization of enrichment and plating procedures for the recovery of Escherichia coli O111 and O26 from minced beef
T.M.G. Catarame
Journal of Applied Microbiology, 95(5) (2003)

Global Trade Item Number

SKUGTIN
76704-500G-F04061832923871