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77515

Sigma-Aldrich

Methylene Blue solution

for microscopy

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Synonym(s):
Ehrlich’s reagent III, Loeffler’s Methylene Blue
Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
Molecular Weight:
319.85
Beilstein:
3641570
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

for microscopy

form

solution

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

suitability

Corynebacterium spp.
bacteria
mycobacteria
spore forming microorganisms

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

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1 of 4

This Item
5048431911248900
form

solution

form

-

form

liquid

form

-

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

limited shelf life

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

-

technique(s)

microbe id | staining: suitable

suitability

Corynebacterium spp., bacteria, mycobacteria, spore forming microorganisms

suitability

Bacillus spp., Corynebacterium spp., bacteria

suitability

-

suitability

Chlamydia spp., Histoplasma spp., Plasmodium spp., Trichomonas spp., bacteria, protozoa, spirochetes

antibiotic activity spectrum

Gram-negative bacteria

antibiotic activity spectrum

-

antibiotic activity spectrum

-

antibiotic activity spectrum

-

Application

Loefflers Methylene Blue stain is used for the identification of diphteria bacilli, since it differentiates the deeply staining metachromatic granules from the pale blue-staining cytoplasm.

Components

Composition:
methylene blue 0.3 g
ethanol 30 ml
water 100 ml

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

113.0 °F

Flash Point(C)

45 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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ZnO1-x/graphene hybrid photocatalyst was prepared via a facile in-situ reduction of graphene oxide and ZnO1-x surface defect oxide. The hybrid photocatlayst showed enhanced photocatalytic activity for the photodegradation of methylene blue. The photocorrosion of ZnO1-x was successfully inhibited by graphene
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To evaluate the diagnostic accuracy of methylene blue used as a non-invasive in vivo stain to detect ocular surface squamous neoplasia (OSSN). A test validation study was performed according to Standards for the reporting of diagnostic accuracy studies (STARD) guidelines
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Some members of the family Enterobacteriaceae ferment sugars via the mixed-acid fermentation pathway. This yields large amounts of acids, causing strong and sometimes even lethal acidification of the environment. Other family members employ the 2,3-butanediol fermentation pathway, which generates comparatively

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