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Sigma-Aldrich

Phosphotungstic acid hydrate

for microscopy

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Synonym(s):
Tungstophosphoric acid
Linear Formula:
H3[P(W3O10)4] · xH2O
CAS Number:
Molecular Weight:
2880.05 (anhydrous basis)
MDL number:
PubChem Substance ID:
NACRES:
MA.02

grade

for microscopy

Quality Level

form

powder or crystals

impurities

≤0.002% total nitrogen (N)

loss

≤15% loss on drying

pH

2 (20 °C)

mp

~95 °C (lit.)

solubility

water: 0.5 g/10 mL, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤100 mg/kg

cation traces

Ca: ≤20 mg/kg
Cu: ≤5 mg/kg
Fe: ≤20 mg/kg
K: ≤50 mg/kg
Na: ≤50 mg/kg
Pb: ≤20 mg/kg
Zn: ≤5 mg/kg

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.OP(O)(O)=O

InChI

1S/H3O4P.H2O.36O.12W/c1-5(2,3)4;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;/h(H3,1,2,3,4);1H2;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;

InChI key

AVFBYUADVDVJQL-UHFFFAOYSA-N

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General description

Phosphotungstic acid hydrate is a heteropolyacid.It is a reagent suitable to detect alkaloids and nitrogenous bases. It is suitable for manufacturing organic pigments, and additives in the plating industry. It is known to impart water resistance to plastics, adhesives, and cement. It also is suitable as a catalyst for organic reactions.

Application

Phosphotungstic acid hydrate may be used as a staining agent for the transmission electron microscope (TEM) imaging of cetyltrimethylammonium bromide (CTAB) coated gold nanorods.It may be used in the preparation of:
  1. Phosphotungstic acid doped ormosil hybrid networks
  2. Niobia supported phosphotungstic acid (PTA) catalysts
  3. Titanium dioxide (TiO2) nanoparticles /phosphotungstic acid (HPW) films.

Other Notes

Review of the folin phenol method for the quantitative determination of proteins

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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G. W. A. Milne
Gardner's Commercially Important Chemicals: Synonyms, Trade Names, and Properties, 496-496 (2005)
Sakari S Karhula et al.
PloS one, 12(1), e0171075-e0171075 (2017-01-31)
Contrast-enhanced micro-computed tomography (CEμCT) with phosphotungstic acid (PTA) has shown potential for detecting collagen distribution of articular cartilage. However, the selectivity of the PTA staining to articular cartilage constituents remains to be elucidated. The aim of this study was to
Tobias Riede et al.
Journal of speech, language, and hearing research : JSLHR, 63(8), 2680-2694 (2020-08-09)
Purpose The larynx plays a role in swallowing, respiration, and voice production. All three functions change during ontogeny. We investigated ontogenetic shape changes using a mouse model to inform our understanding of how laryngeal form and function are integrated. We
Tony Werner et al.
Scientific reports, 8(1), 5465-5465 (2018-04-05)
The most common allergen in fish, the highly-abundant protein β-parvalbumin, forms amyloid structures as a way to avoid gastrointestinal degradation and transit to the blood. In humans, the same amyloid structures are mostly associated with neurodegenerative disorders such as Alzheimer's
Todd A Schoborg
Journal of visualized experiments : JoVE, (163) (2020-09-22)
Biomedical imaging tools permit investigation of molecular mechanisms across spatial scales, from genes to organisms. Drosophila melanogaster, a well-characterized model organism, has benefited from the use of light and electron microscopy to understand gene function at the level of cells

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