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Merck
CN

82940

3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt

for spectrophotometric det. of Fe, ≥99.0%

Synonym(s):

5,5′-[3-(2-Pyridyl)-1,2,4-triazine-5,6-diyl]difuran-2-sulfonic acid disodium salt

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About This Item

Empirical Formula (Hill Notation):
C16H8N4Na2O8S2
CAS Number:
79551-14-7
Molecular Weight:
494.37
UNSPSC Code:
12352200
NACRES:
NA.21
PubChem Substance ID:
329769271
MDL number:
MFCD00040642
Beilstein/REAXYS Number:
5710932

Key Documents

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Product Name

3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt, for spectrophotometric det. of Fe, ≥99.0%

InChI

1S/C16H10N4O8S2.2Na/c21-29(22,23)12-6-4-10(27-12)14-15(11-5-7-13(28-11)30(24,25)26)19-20-16(18-14)9-3-1-2-8-17-9;;/h1-8H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2

SMILES string

[Na+].[Na+].[O-]S(=O)(=O)c1ccc(o1)-c2nnc(nc2-c3ccc(o3)S([O-])(=O)=O)-c4ccccn4

InChI key

IVLSEFOVPQFJBB-UHFFFAOYSA-L

assay

≥99.0% (TLC)
≥99.0%

form

crystals

quality

for spectrophotometric det. of Fe

technique(s)

UV/Vis spectroscopy: suitable

Quality Level

100

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Application

It was used in chromogen screening while studying non-transferrin-bound iron quantification. It was used in measuring the chelating activity on ferrous ions.

General description

3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt also known as Ferene is a chelating agent which usually binds with Fe2+ ion to form coloured complex.

Other Notes

Colorimetric reagent for iron, which is more sensitive than other reagents (e.g. Ferrozine), molar absorptivity 35500 liters/mol cm

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN9759
BCCN9275
BCCN3166
BCCM6745
BCCL3263

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Microchemical Journal, Devoted to the Application of Microtechniques in All Branches of Science, 28, 275-275 (1983)
D.J. Hennessy et al.
Canadian Journal of Chemistry, 62, 721-721 (1984)
Interaction between bovine control materials and the ferene iron method.
H M Barbour et al.
Annals of clinical biochemistry, 30 ( Pt 2), 221-222 (1993-03-01)
S Eskelinen et al.
Scandinavian journal of clinical and laboratory investigation, 43(5), 453-455 (1983-09-01)
Ferene-S was tested as the iron chromogen and the results were compared with the chromogen Ferrozine. With Ferene-S the volume of serum could be halved. We also tried to adapt Ferene-S in total iron binding capacity determinations but failed. From
M C Chung
Analytical biochemistry, 148(2), 498-502 (1985-08-01)
A new method for specifically staining the iron atoms present in transferrin and lactoferrin after polyacrylamide gel electrophoresis and isoelectric focusing is described. The stain, 3-(2-pyridyl)-5,6-bis(2-(5-furylsulfonic acid))-1,2,4-triazine, disodium salt, or Ferene S, will detect transferrin in 5 microliters of human
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