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Merck
CN

82950

3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt

for spectrophotometric det. of Fe, 97.0%

Synonym(s):

5,6-Diphenyl-3-(2-pyridyl)-1,2,4-triazine-4′,4″-disulfonic acid sodium salt, PDT disulfonate

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About This Item

Empirical Formula (Hill Notation):
C20H13N4NaO6S2
CAS Number:
69898-45-9
Molecular Weight:
492.46
NACRES:
NA.21
PubChem Substance ID:
329769272
UNSPSC Code:
12352200
EC Number:
274-196-3
MDL number:
MFCD00078417

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Product Name

3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt, for spectrophotometric det. of Fe, 97.0%

InChI key

ZGVNYCXXBQPDPQ-UHFFFAOYSA-M

InChI

1S/C20H14N4O6S2.Na/c25-31(26,27)15-8-4-13(5-9-15)18-19(14-6-10-16(11-7-14)32(28,29)30)23-24-20(22-18)17-3-1-2-12-21-17;/h1-12H,(H,25,26,27)(H,28,29,30);/q;+1/p-1

SMILES string

[Na+].OS(=O)(=O)c1ccc(cc1)-c2nnc(nc2-c3ccc(cc3)S([O-])(=O)=O)-c4ccccn4

assay

97.0%

form

solid

quality

for spectrophotometric det. of Fe

technique(s)

UV/Vis spectroscopy: suitable

impurities

~7% water

mp

≥300 °C

cation traces

Co: ≤50 mg/kg
Cu: ≤50 mg/kg
Fe: ≤50 mg/kg
Mo: ≤50 mg/kg
Ni: ≤50 mg/kg
V: ≤50 mg/kg
Zn: ≤50 mg/kg

Quality Level

100

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Application

It was used in determining of chelating activity of Fe2+ using Boyer and McCleary method.

General description

3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt also known as Ferrozine is a chelating agent for ferrous iron.

Other Notes

Highly specific water-soluble reagent for the accurate determination of submicrogram levels of serum iron (pH 3.5-4.5, lmax 562 nm, A 29′000)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP0657
BCCP0386
BCCN7114
WXBF6586V
BCCN4669

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K.L. Stookey
Analytical Chemistry, 42, 779-779 (1970)
Molecular characterization of a putative Arabidopsis thaliana copper transporter and its yeast homologue.
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The Journal of Biological Chemistry, 270.47, 28479-28486 (1995)
Antioxidative activity of protein hydrolysate from round scad muscle using alcalase and flavourzyme.
Thiansilakul, Yaowapa, Soottawat Benjakul, and Fereidoon Shahidi.
Journal of Food Biochemistry, 31.2 , 266-287 (2007)
Direct and indirect assays of serum iron with a sensitive new color reagent.
D Plaut et al.
The American journal of medical technology, 38(3), 82-89 (1972-03-01)
F Ceriotti et al.
Clinical chemistry, 26(2), 327-331 (1980-02-01)
Serum iron is released from transferrin and reduced at pH 1.7 by treating serum with a 10 g/L ascorbic acid solution in 0.1 mol/L HCl. When ferrozine is added to this reagent, it forms a complex with iron that is
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