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857343

Basic Fuchsin

certified by the BSC, Dye content ≥88 %

Synonym(s):

Basic Parafuchsin, Basic Red 9, Magenta™ O, Parafuchsin hydrochloride, Paramagenta hydrochloride, Pararosaniline chloride, Pararosaniline hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C19H17N3 · HCl
CAS Number:
569-61-9
Molecular Weight:
323.82
NACRES:
NA.47
PubChem Substance ID:
24888442
UNSPSC Code:
12171500
Colour Index Number:
42500
EC Number:
209-321-2
MDL number:
MFCD00001657
Beilstein/REAXYS Number:
4164603
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agency

certified by the BSC

Quality Level

200

form

powder

composition

Dye content, ≥88%

technique(s)

microbe id | staining: suitable

color

green to dark green

pH

1.0-3.1, purple to red

mp

268-270 °C (dec.) (lit.)

λmax

544 nm

ε (extinction coefficient)

≥11000 at 235-239 nm in 50% ethanol at 0.003 g/L, ≥17000 at 287-291 nm in 50% ethanol at 0.003 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Nc1ccc(cc1)C(\c2ccc(N)cc2)=C3/C=CC(=[NH2+])C=C3

InChI

1S/C19H17N3.ClH/c20-16-7-1-13(2-8-16)19(14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15;/h1-12,20H,21-22H2;1H

InChI key

JUQPZRLQQYSMEQ-UHFFFAOYSA-N

Application

Basic fuchsin has been used as a lignin-detecting fluorogenic dye for fluorescence-based imaging of lignin in the basal internodes of three Brachypodium distachyon ecotypes.
Certified for use for acid-fast staining with carbol-fuchsin, as ENDO medium, and as Schiff′s reagent in the Periodic Acid Schiff (PAS) and Feulgen techniques.

Biochem/physiol Actions

Basic fuchsin belongs to the triphenylmethane group. Combining basic fuchsin with sulfurous acid results in the formation of fuchsin sulfurous acid, also referred to as Feulgen reagent or Schiff′s reagent. This reagent is responsible for the staining of chromosomal DNA. Basic fuchsin is also used for the staining of lignin in plant tissue samples.

Legal Information

Magenta is a trademark of Merck KGaA, Darmstadt, Germany

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT6874
SHBT3375
SHBS5612
SHBR3338
SHBS0115

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Min Chen et al.
The New phytologist, 229(6), 3481-3496 (2020-11-25)
The intimate association of host and fungus in arbuscular mycorrhizal (AM) symbiosis can potentially trigger induction of host defence mechanisms against the fungus, implying that successful symbiosis requires suppression of defence. We addressed this phenomenon by using AM-defective vapyrin (vpy)
Bisen PS
Laboratory Protocols in Applied Life Sciences (2014)
Plant Fibers (2012)
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Global Trade Item Number

SKUGTIN
857343-100G04061833032244
857343-25G04061833032251