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Merck
CN

85766

m-Endo Agar LES

NutriSelect® Plus, powder, selective for coliforms, pack of 500 g, suitable for microbiology

Synonym(s):

Membrane Endo Agar LES

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171606
Manufacturer/tradename:
NutriSelect® Plus
Form:
powder
Suitability:
selective and differential for Escherichia coli, selective and differential for Proteus spp., selective and differential for Salmonella spp., selective for coliforms
Application(s):
agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology
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sterility

non-sterile

form

powder

Quality Level

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L , casein hydrolysate, 3.7 g/L , di-potassium hydrogen phosphate, 3.3 g/L , lactose, 9.4 g/L , pararosanilin (fuchsin), 0.8 g/L , peptone from meat, 3.7 g/L , potassium hydrogen phosphate, 1 g/L , sodium chloride, 3.7 g/L , sodium deoxycholate, 0.1 g/L , sodium lauryl sulfate, 0.05 g/L , sodium sulfite, 1.6 g/L , tryptose, 7.5 g/L , yeast extract, 1.2 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.2±0.2±25 °C

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology

suitability

selective and differential for Escherichia coli, selective and differential for Proteus spp., selective and differential for Salmonella spp., selective for coliforms

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Application

m-Endo Agar LES can be used for the enumeration of coliforms in water using the Membrane filter technique for coliform enumeration. This method is more reliable and precise than MPN multiple test. It follows the two step membrane filter procedure using Lauryl Sulfate Broth (Cat. No. 17349) as a preliminary enrichment, resulting in higher coliform counts.

Preparation Note

Suspend 51 g in 1 litre of distilled water containing 20 ml of ethanol 96 %. Boil to dissolve completely. Do not autoclave! Cool to 45-50°C and dispense 4 ml amounts into 50-60 mm Petri dishes and allow to solidify. The agar should be at least 1.5mm depth. Do not expose plates to direct sunlight.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Information

动植物来源培养基
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Ronald M. Atlas
Handbook of Microbiological Media null

Articles

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

有关用于分化和检测大肠杆菌和其他大肠菌群的选择性生长培养基的一篇文章。

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