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Merck
CN

860867

Brilliant Cresyl Blue ALD

Certified by the Biological Stain Commission

Synonym(s):

3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1), Brilliant Blue C

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About This Item

Empirical Formula (Hill Notation):
C17H20ClN3O · 0.5ZnCl2
CAS Number:
51716-96-2
Molecular Weight:
385.96
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24888521
EC Number:
279-675-0
Beilstein/REAXYS Number:
5690713
MDL number:
MFCD00148901

Key Documents

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Product Name

Brilliant Cresyl Blue ALD, Certified by the Biological Stain Commission

InChI key

SIHASHFMDFXGSX-UHFFFAOYSA-J

InChI

1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.CC\[N+](CC)=C1\C=CC2=Nc3cc(C)c(N)cc3OC2=C1.CC\[N+](CC)=C4\C=CC5=Nc6cc(C)c(N)cc6OC5=C4

form

powder or crystals

composition

Dye content, ≥50%

technique(s)

microbe id | staining: suitable

mp

233-236 °C (lit.)

λmax

622 nm

ε (extinction coefficient)

≥20000 at 237-241 nm in 50% ethanol at 0.005 g/L
≥20000 at 248-252 nm in 50% ethanol at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Brilliant cresyl blue ALD has been used for the staining of reticulocytes.

Biochem/physiol Actions

Brilliant cresyl blue ALD is responsible for the staining of RNA in reticulocytes.

Other Notes

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS7555
SHBR3592
SHBS0804
SHBR5385
SHBP9883

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Inflammatory and oxidative stress phenotypes in transgenic sickle cell mice.
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Blood Cells, Molecules and Diseases, 62, 13-13 (2016)
Pal GK and Pal P
Textbook Of Practical Physiology (2006)
Clark, G., Ed.
Staining Procedures, 176-176 (1981)
A Zh Baltabekova et al.
Molecular biotechnology, 57(8), 746-755 (2015-04-04)
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