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Merck
CN

861006

Eosin B

certified by the BSC, Dye content 90 %

Synonym(s):

4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt, Acid Red 91, Eosin Scarlet, Eosin bluish, Saffrosine

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About This Item

Linear Formula:
C20H6N2O9Br2Na2
CAS Number:
548-24-3
Molecular Weight:
624.06
Colour Index Number:
45400
NACRES:
NA.47
PubChem Substance ID:
24888525
UNSPSC Code:
12171500
EC Number:
208-943-1
MDL number:
MFCD00005041
Beilstein/REAXYS Number:
4121964

Key Documents

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Product Name

Eosin B, certified by the BSC, Dye content 90 %

InChI key

GYYTYUGGVBYJHE-UHFFFAOYSA-L

InChI

1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

SMILES string

[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O

agency

certified by the BSC

form

powder

composition

Dye content, 90%

technique(s)

microbe id | staining: suitable

solubility

H2O: 1 mg/mL, clear, red

λmax

395 nm
514 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

  • Eosin B has been used for the histological analysis of anal sphincter sections obtained from rats.
  • It has been used along with Mayer′s hematoxylin for the staining of triangularis sterni muscle sections.
  • It has been used along with hematoxylin for the staining of different tissue sections obtained from mouse.
Counterstain for collagen, following Mayer′s Hemalum. Used with Azure A as a tissue stain for cell granules, nuclei, and microorganisms. For differential staining of cells of the anterior pituitary.

Biochem/physiol Actions

Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer′s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining of cell granules, nuclei and microorganisms. It can also be used for protein estimation. The protein - eosin B complex absorbs at wavelength range of 536–544nm and the change in blue shift helps in determining the protein concentration.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBQ9180
MKCJ7016
MKCH8108
MKBL4929V
MKAA2519V

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Restoration of anal sphincter function after myoblast cell therapy in incontinent rats.
Bisson A, et al.
Cell Transplantation, 24, 277-277 (2015)
Bone marrow deficiency of MCPIP1 results in severe multi-organ inflammation but diminishes atherogenesis in hyperlipidemic mice.
Yu F, et al.
PLoS ONE, 8, e80089-e80089 (2013)
The influence of passive stretch and NF-?B inhibitors on the morphology of dystrophic muscle fibers.
Siegel AS, et al.
Anatomical Record (Hoboken, N.J. : 2007), 294, 132-132 (2011)
Estimation of protein using eosin B dye.
Waheed AA and Gupta PD
Analytical Biochemistry, 233, 249-249 (1996)
Theory and Practice of Histological Techniques (2008)
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