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861111

Sigma-Aldrich

Bismarck Brown Y

certified by the Biological Stain Commission, Dye content 50 %

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Synonym(s):
4,4′-(m-Phenylenebisazo)bis-m-phenylenediamine dihydrochloride, Basic Brown 1, Bismarck Brown B, Vesuvine
Empirical Formula (Hill Notation):
C18H18N8 · 2HCl
CAS Number:
Molecular Weight:
419.31
Colour Index Number:
21000
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, 50%

solubility

H2O: 10 mg/mL, clear to turbid, red-orange to red

λmax

457 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl.Cl.Nc1ccc(\N=N\c2cccc(c2)\N=N\c3ccc(N)cc3N)c(N)c1

InChI

1S/C18H18N8.2ClH/c19-11-4-6-17(15(21)8-11)25-23-13-2-1-3-14(10-13)24-26-18-7-5-12(20)9-16(18)22;;/h1-10H,19-22H2;2*1H/b25-23+,26-24+;;

InChI key

MCZVRBLCRZWFJH-SPBSJSFYSA-N

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1 of 4

This Item
861006857343A3157
Bismarck Brown Y certified by the Biological Stain Commission, Dye content 50 %

Sigma-Aldrich

861111

Bismarck Brown Y

Eosin B certified by the Biological Stain Commission, Dye content 90 %

Sigma-Aldrich

861006

Eosin B

Basic Fuchsin certified by the Biological Stain Commission, Dye content ≥88 %

Sigma-Aldrich

857343

Basic Fuchsin

Alcian Blue 8GX certified by the Biological Stain Commission

Sigma-Aldrich

A3157

Alcian Blue 8GX

form

powder

form

powder

form

powder

form

powder

composition

Dye content, 50%

composition

Dye content, 90%

composition

Dye content, ≥88%

composition

Dye content, ≥50%

solubility

H2O: 10 mg/mL, clear to turbid, red-orange to red

solubility

H2O: 1 mg/mL, clear, red

solubility

-

solubility

water: 1 mg/mL, blue

λmax

457 nm

λmax

395 nm, 514 nm

λmax

544 nm

λmax

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

Application

Bismarck Brown Y is used for the staining of plant samples, including stem, root, starch granules and epidermis.

Suitability

Certified for use in staining goblet cells and cartilage.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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The Journal of Laboratory and Clinical Medicine, 26, 1200-1200 (1941)
R.D. Lillie Ed.
Conn?s Biological Stains, 145-145 (1977)
The use of Bismarck brown Y in some new stain schedules.
Blaydes GW
Stain Technology, 14.3, 105-110 (1939)
E T Graham et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 73(4), 178-185 (1998-09-15)
A staining solution containing alcian blue 8GX, Bismarck brown Y and safranin O was prepared with 0.1 M sodium acetate buffer, pH 5.0. Paraffin was extracted with MicroClear solvent from 10 microm tissue sections mounted on slides. Paraffin solvent was
B G Prakash Kumar et al.
Journal of hazardous materials, 136(3), 922-929 (2006-03-03)
Activated carbon was produced from a biowaste product, rubberwood sawdust (RWSD) using steam in a high temperature fluidized bed reactor. Experiments were carried out to investigate the influence of various process parameters such as activation time, activation temperature, particle size

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