89823
ECC ChromoSelect Selective Agar
suitable for microbiology
Synonym(s):
E. coli-Coliform Selective Agar ChromoSelect, ECC Selective Agar ChromoSelect
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About This Item
UNSPSC Code:
12352200
MDL number:
shelf life
limited shelf life, expiry date on the label
composition
agar, 10 g/L , casein enzymic hydrolysate, 3.3 g/L , chromogenic mixture, 0.43 g/L , disodium hydrogen phosphate, 1 g/L , peptone, special, 6 g/L , sodium chloride, 2 g/L , sodium dihydrogen phosphate, 0.60 g/L , sodium pyruvate, 1 g/L , sorbitol, 1 g/L , Tergitol™
7, 0.15 g/L , tryptophan, 1 g/L
final pH
6.8±0.2 (25 °C)
application(s)
microbiology
Application
HiCrome™ ECC Selective Agar is a selective medium recommended for the simultaneous detection of Escherichia coli and coliforms in water and food samples. The ingredients help even the sublethally injured coliforms to grow rapidly, Tergitol™ inhibits Gram-positive as well as some Gram-negative bacteria other than coliforms. The mixture contains two chromogenic substrates as Salmon-GAL and X-glucuronide. The enzyme β-D-galactosidase produced by coliforms cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by Escherichia coli, cleaves X-glucuronide. Escherichia coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction. To confirm E. coli, add a drop of Kovac′s reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.
Preparation Note
Suspend 26.5 g in 1 liter of distilled water. Heat in a boiling water bath or in free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance.
If desired to inhibit Pseudomonas and Aeromonas species, add 5 mg of Cefsulodin for surface and pour plate method and 10 mg of Cefsulodin for membrane filter technique. Mix well.
If desired to inhibit Pseudomonas and Aeromonas species, add 5 mg of Cefsulodin for surface and pour plate method and 10 mg of Cefsulodin for membrane filter technique. Mix well.
Legal Information
HiCrome is a trademark of HiMedia Laboratories Pvt. Ltd.
TERGITOL is a trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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