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Merck
CN

91366

Universal Pre-Enrichment Broth

NutriSelect® Plus, powder, nonselective for Salmonella spp., for Listeria spp., pack of 500 g, suitable for microbiology

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Plus
Form:
powder
Suitability:
Listeria spp., nonselective for Salmonella spp.
Application(s):
environmental
food and beverages
sample preparation, microbiology

Key Documents

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sterility

non-sterile

form

powder

Quality Level

200

shelf life

limited shelf life, expiry date on the label

composition

casein enzymic hydrolysate, 5.0 g/L , dextrose, 0.50 g/L , disodium phosphate, 7.0 g/L , ferric ammonium citrate, 0.10 g/L , magnesium sulfate, 0.250 g/L , monopotassium phosphate, 15.0 g/L , proteose peptone, 5.0 g/L , sodium chloride, 5.0 g/L , sodium pyruvate, 0.20 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.3±0.2 (25 °C)

application(s)

environmental
food and beverages
sample preparation, microbiology

suitability

Listeria spp., nonselective for Salmonella spp.

Related Categories

General description

Universal Pre-enrichment Broth (UPB) is used for recovering sublethally injured Salmonella and Listeria from food products. UPB lacks inhibitory agents to provide selectivity for target pathogens and may not be adapted for samples with high levels of background microflora.

Application

Universal Pre-enrichment Broth (UPB) has been used in the enrichment of Listeria monocytogenes and Salmonella species. It has also been used in the growth kinetic studies of Salmonella typhimurium.

Preparation Note

Suspend 38.05 grams in 1000 ml distilled water. Heat if necessary, to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN5762
BCCN0659
BCCM3714
BCCL1566
BCCK9347

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Ratthaphol Charlermroj et al.
International journal of food microbiology, 224, 47-54 (2016-03-08)
This study rigorously evaluated a previously developed immunobead array method to simultaneously detect three important foodborne pathogens, Campylobacter jejuni, Listeria monocytogenes, and Salmonella spp., for its actual application in routine food testing. Due to the limitation of the detection limit
Qianwang Zheng et al.
Food microbiology, 52, 159-168 (2015-09-05)
The ability of nine commercial broths to enrich healthy and 90% sanitizer-injured Salmonella Typhimurium and Salmonella cocktail on mung bean sprouts was evaluated to select an optimum broth for detection. Results showed that S. Typhimurium multiplied faster and reached a

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