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Merck
CN

92435

TBX Agar

NutriSelect® Plus, powder, for Escherichia coli, pkg of 100 g, pkg of 500 g

Synonym(s):

Tryptone Bile X-glucuronide Agar, Chromogenic E. coli Agar, Chromogenic Tryptone Bile Glucuronide Agar, Tryptone Bile X-Glucuronide Agar, Tryptone-bile-glucuronic medium

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171606

Key Documents

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Product Name

TBX Agar, NutriSelect® Plus, powder, for Escherichia coli, pkg of 100 g, pkg of 500 g

agency

according to ISO 13136:2012
according to ISO 16649
according to ISO 9308-4:2025

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
bile salts, 1.5 g/L
X-β-D-glucuronide, 0.075 g/L
peptone, 20 g/L

packaging

pkg of 100 g
pkg of 500 g

manufacturer/tradename

NutriSelect® Plus

final pH

7.2±0.2 (25 °C in H2O)

application(s)

agriculture
bioburden testing
environmental
food and beverages
microbiology
water monitoring

suitability

Escherichia coli
selective and differential for coliforms

Quality Level

200

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 36.6 g in 1 litre distilled water. Heat in a boiling water bath or in flowing steam until the medium is completely dissolved. Autoclave at 121°C for 15 min. Cool to 45-50°C in a water bath, mix gently and pour 15 ml in sterile petri dishes. If stored at +2 to +8°C and protected from light the plates are stable for 1 week.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

TBX Agar is widely used for the enumeration, detection, and identification of β-glucuronidase positive E. coli from food, feeding stuff, and environmental samples in food production and handling. It is particularly effective in differentiating E. coli from other coliforms, making it a valuable tool in microbiological testing for food safety and water monitoring.

Features and Benefits

NutriSelect® Plus is a powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

General description

TBX Agar (Tryptone Bile X-glucuronide Agar) is a chromogenic medium designed for the selective detection and enumeration of Escherichia coli in foodstuffs, animal feed, and water. E coli cells absorb x-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives blue to blue-green coloration to the colonies. The peptone provides the essential growth nutrients to the organisms. Bile salts mixture inhibits Gram-positive organisms and the high incubation temperature of 44 °C further suppresses non-target organisms.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN8545
BCCM8979
BCCL8835
BCCL2261
BCCJ9864

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Articles

Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

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有关用于分化和检测大肠杆菌和其他大肠菌群的选择性生长培养基的一篇文章。

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