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Merck
CN

95388

Bismuth Sulfite Agar

NutriSelect® Basic, according to GB 4789.4-2016, powder, selective for Salmonella spp., pack of 500 g, suitable for microbiology

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171606
Manufacturer/tradename:
NutriSelect® Basic
Agency:
according to GB 4789.4-2016
Form:
powder
Suitability:
selective for Salmonella spp.
Application(s):
clinical testing
environmental
food and beverages, microbiology

Key Documents

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agency

according to GB 4789.4-2016

sterility

non-sterile

form

powder

Quality Level

100

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L , bismuth ammonium citrate, 1.85 g/L , brilliant green, 0.025 g/L , disodium hydrogen phosphate, 4 g/L , D(+)-glucose, 5 g/L , iron(II) sulfate 7x H2O, 0.55 g/L (equivalentto 0.3g/L iron(ll) sulfate water free), meat extract, 5 g/L , mixed peptone, 10 g/L , sodium sulfite, 6.15 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.6±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages, microbiology

suitability

selective for Salmonella spp.

Related Categories

Application

Selective medium for the isolation and differentiation of Salmonella typhi and other salmonellae from clinical material etc.
The prepared agar is turbid and greenish-yellow.

Preparation Note

Dissolve 47.55 g in litre distilled water and heat to boiling to dissolve the medium completely. DO NOT STERILIZE. This medium should be prepared, and the plates poured, 24h in advance of their intended use, since the freshly prepared medium is excessively toxic to Salmonellae. It must also be remembered the medium loses selectivity after 3-4 days, even when refrigerated.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

hcodes

H412

Hazard Classifications

Aquatic Chronic 3

supp_hazards

EUH031

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

监管及禁止进口产品
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN8337
BCCN3786
BCCN0513
BCCM3359
BCCL5532

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Further Experience of the Bismuth Sulphite Media in the Isolation of Bacillus typhosus and B. paratyphosus B from Faeces, Sewage and Water.
W J Wilson et al.
The Journal of hygiene, 31(2), 138-161 (1931-04-01)
Use of a Glucose Bismuth Sulphite Iron Medium for the Isolation of B. typhosus and B. proteus.
W J Wilson et al.
The Journal of hygiene, 26(4), 374-391 (1927-10-01)
J.H. MacCoy
The Journal of Applied Bacteriology, 25, 213-213 (1962)

Articles

Microbiological Control of Spices and Herbs

Microbiological assessment of bacteria found in spices, a potent source for food spoilage and pathogens.

Differentiation and ID Media for Salmonella

Salmonella, with 2,300+ serotypes, causes half of food-borne illnesses, often from dairy, poultry, and eggs.

沙门氏菌分离和鉴定培养基

沙门氏菌污染是全球食源性疾病的第二大成因。控制沙门氏菌的暴发是食品监管机构、餐馆和整个食品工业的一项重要任务。沙门氏菌家族包括2300多种血清型的细菌，但其中有两种类型，即肠炎沙门氏菌和鼠伤寒沙门氏杆菌，约占所有人类感染的一半。大多数沙门氏菌的暴发可以追溯到乳制品、家禽和肉制品，但沙门氏菌几乎可以在任何食物上生长。鸡、鸡蛋及其衍生产品的风险特别高。

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