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95388

Bismuth Sulfite Agar

NutriSelect® Basic, according to GB 4789.4-2016, powder, selective for Salmonella spp., pack of 500 g, suitable for microbiology

Synonym(s):

Salmonella Selective Agar

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171606
eCl@ss:
32160607
Manufacturer/tradename:
NutriSelect® Basic
Agency:
according to GB 4789.4-2016
Form:
powder
Suitability:
selective for Salmonella spp.
Application(s):
clinical testing
environmental
food and beverages, microbiology
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agency

according to GB 4789.4-2016

Quality Segment

sterility

non-sterile

product line

NutriSelect® Basic

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L , bismuth ammonium citrate, 1.85 g/L , brilliant green, 0.025 g/L , disodium hydrogen phosphate, 4 g/L , D(+)-glucose, 5 g/L , iron(II) sulfate 7x H2O, 0.55 g/L (equivalentto 0.3g/L iron(ll) sulfate water free), meat extract, 5 g/L , mixed peptone, 10 g/L , sodium sulfite, 6.15 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

color

greenish yellow

final pH

7.6±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages, microbiology

suitability

selective for Salmonella spp.

General description

Bismuth Sulfite Agar is a highly selective medium designed for the isolation and detection of Salmonella species, including Salmonella typhi, from food, water, and clinical samples. The medium′s selectivity is achieved through the combined action of freshly precipitated bismuth sulfite and brilliant green, which effectively suppresses the growth of coliforms while permitting the growth of salmonellae. Sulfur compounds in the medium provide a substrate for hydrogen sulfide production, and the metallic salts, stain the colonies and surrounding medium black or brown in the presence of hydrogen sulfide, providing a clear visual indicator for Salmonella identification.

Application

Bismuth Sulfite Agar is used for the selective isolation and detection of Salmonella species in food, water, and clinical specimens. The medium is particularly valuable in food safety and clinical microbiology laboratories where the detection of Salmonella is critical. When the sample contains high levels of organic matter, it is recommended to suspend the sample in sterile saline and use the supernatant for inoculation to prevent the formation of atypical colonies.

Features and Benefits

NutriSelect® Basic is a powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Preparation Note

Dissolve 47.55 g in litre distilled water and heat to boiling to dissolve the medium completely. DO NOT STERILIZE. This medium should be prepared, and the plates poured, 24h in advance of their intended use, since the freshly prepared medium is excessively toxic to Salmonellae. It must also be remembered the medium loses selectivity after 3-4 days, even when refrigerated.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product will be discontinued in the near future; however, stock is still available. The recommended replacement product is 1.00191 - Bismuth Sulfite (BS) Agar

Hazard Codes

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

supp_hazards

EUH031

Storage Class

11 - Combustible Solids

WGK

WGK 3

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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