Mueller-Hinton Agar 2

Mueller-Hinton Agar 2 is a selective medium recommended for the disk diffusion method of antibiotic susceptibility testing. It is formulated for testing rapidly growing aerobic or facultatively anaerobic bacterial pathogens. The medium is nutritionally supported by beef heart infusion and acid casein hydrolysate, which provide essential nitrogenous compounds, vitamins, carbon, sulfur, amino acids, and other growth nutrients. Starch is incorporated into the formulation as a protective colloid against toxic substances present in the medium. A key feature of Mueller Hinton Agar 2 is its controlled composition: it is manufactured to contain low levels of thymine and thymidine, which are known to inhibit sulfonamide and trimethoprim activity, and controlled levels of calcium and magnesium, which can interfere with the activity of aminoglycoside antibiotics. This precise formulation ensures reliable and reproducible antimicrobial susceptibility test results.

Mueller Hinton Agar 2 is widely used in clinical and diagnostic microbiology laboratories for antimicrobial susceptibility testing of bacterial pathogens using the disk diffusion method as recommended by Bauer et al. and NCCLS. The medium is commonly used in food and beverage, pharmaceutical, and environmental monitoring industries, where reliable and consistent microbial growth is essential for accurate results.

Features:

• High-Quality Standards: NutriSelect^® Plus powdered culture media are made from carefully selected raw materials, ensuring consistent quality.
• Wide Range of Applications: Suitable for various microbiological needs, including academic research, food and beverage testing, and pharmaceutical applications.
• Regulatory Compliance: Products meet industry standards and are compliant with ISO 9001 quality assurance.
• Innovative Formulations: Includes novel media types such as non-animal-derived alternatives, synthetically defined media, and chromogenic media.

Benefits:

• Convenience and Safety: Easy-to-use powdered format with minimal clumping and excellent solubility, even in warm or humid conditions.
• Sustainability: Reduced risks associated with fine dust and toxic substance inhalation, ensuring a safer work environment.
• Extended Shelf Life: Products have a prolonged shelf life of up to five years.
• High Reproducibility: Ensures consistent results across batches, exceeding regulatory demands.
• Comprehensive Portfolio: A vast selection of over 350 products tailored to meet diverse microbiological testing needs.

Suspend 38 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 50°C. Mix gently and dispense into sterile Petri dishes.

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany