A3723
ADVASEP™-7
solid
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About This Item
Linear Formula:
C42H70-nO35(C4H6SO3Na)n, avg. n = 6.5
NACRES:
NA.47
UNSPSC Code:
12171500
form
solid
Quality Level
mol wt
~2163
color
white
solubility
H2O: soluble
application(s)
hematology
histology
storage temp.
−20°C
Application
ADVASEP™-7 has been used in washing solution to remove excess dyes, FM4-64FX and FM1-43, in stained cells.
Biochem/physiol Actions
ADVASEP™-7 is a β-cyclodextran derivative. It works as a dye scavenger and removes the background dye.
Reduces background staining of plasma membrane with SynaptoGreen™.
Other Notes
Sulfonated β-cyclodextrin. Component of NTSI, NTSII, and NTSV, also sold individually.
Legal Information
Advasep is a trademark of Cydex Pharmaceuticals, Inc.
SynaptoGreen is a trademark of Biotium, Inc.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Imaging synaptic vesicle exocytosis and endocytosis with FM dyes.
Gaffield MA and Betz WJ
Nature Protocols, 1, 2916-2916 (2006)
A R Kay et al.
Neuron, 24(4), 809-817 (2000-01-07)
The fluorescent probe FM1-43 has been used extensively for imaging vesicle recycling; however, high nonspecific adsorption resulting in elevated background levels has precluded its use in certain tissues, notably brain slices. We have found that a sulfobutylated derivative of beta-cyclodextrin
The Power of Human Protective Modifiers: PLS3 and CORO1C Unravel Impaired Endocytosis in Spinal Muscular Atrophy and Rescue SMA Phenotype.
Hosseinibarkooie S, et al.
American Journal of Human Genetics, 99, 647-647 (2016)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A3723-250MG | 04061832339900 |