A5533
Alizarin Red S
certified by the Biological Stain Commission
grade
certified by the Biological Stain Commission
Quality Level
form
powder
concentration
0.015 g/L in 0.1 M NaOH
technique(s)
titration: suitable
solubility
H2O: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].Oc1c(O)c(cc2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O
InChI
1S/C14H8O7S.Na/c15-11-6-3-1-2-4-7(6)12(16)10-8(11)5-9(22(19,20)21)13(17)14(10)18;/h1-5,17-18H,(H,19,20,21);/q;+1/p-1
InChI key
HFVAFDPGUJEFBQ-UHFFFAOYSA-M
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Related Categories
Application
- Alizarin Red S has been used for checking the calcium deposits in osteogenic culture.
- It has been used for the quantitative analyses of calcification in aortic explants.
- It has been used for mineral staining in osteoblast cultures.
Biochem/physiol Actions
Suitability
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
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Product A5533, Alizarin Red S, is commonly used to stain calcium in tissue sections. Will other cations such as magnesium, manganese, barium and strontium also be detected?
No, typically only calcium is present in sufficient quantities to be demonstrated in tissue sections.
Product A5533, Alizarin Red S, has been prepared according to protocol. The solution has gelled or thickened. Is the solution still usable?
Yes. Although the solution has thickened or gelled, it is still suitable for use.
I see that Product A5533, Alizarin Red S, was tested according to the Dahl method at pH 7.0. Can the dye also be used in the McGee-Russell procedure at pH 4.2?
Yes. The dye can be used at a variety of pH values.
What are the pH transition ranges for Product A5533, Alizarin Red S?
Although the pH transition ranges are not determined on a lot to lot basis, the solution changes from yellow to red in the range of 3.5-6.5, and from the orange to violet at pH 9.4-12. On a lot to lot basis there can be considerable difference in regards to color and pH ranges.
When using Product A5533, Alizarin Red S, will amorphous calcium deposits be detected in tissue sections?
Amorphous calcium phosphate deposits are very soluble below pH 8.5. In most instances the deposits are lost during either standard fixation or the during the standard staining procedure.
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