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Merck
CN

A5882

Antipyrine

analytical standard

Synonym(s):

2,3-Dimethyl-1-phenyl-3-pyrazolin-5-one, Phenazone

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About This Item

Empirical Formula (Hill Notation):
C11H12N2O
CAS Number:
60-80-0
Molecular Weight:
188.23
UNSPSC Code:
41116107
NACRES:
NA.24
PubChem Substance ID:
24891029
EC Number:
200-486-6
Beilstein/REAXYS Number:
157775
MDL number:
MFCD00003146

Key Documents

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Product Name

Antipyrine, analytical standard

InChI key

VEQOALNAAJBPNY-UHFFFAOYSA-N

InChI

1S/C11H12N2O/c1-9-8-11(14)13(12(9)2)10-6-4-3-5-7-10/h3-8H,1-2H3

SMILES string

CN1N(C(=O)C=C1C)c2ccccc2

grade

analytical standard

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

mp

109-111 °C (lit.)

application(s)

forensics and toxicology
pharmaceutical (small molecule)

format

neat

Quality Level

100

Gene Information

human ... PTGS1(5742)

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Application

Antipyrine may be used as:
  • Pharmaceutical reference standard for the determination of the analyte in animal plasma and serum by various chromatography techniques.
  • Internal standard for the analysis of creatinine and uric acid in human plasma by micellar electrokinetic chromatography.
  • Reference standard to investigate the effect of diseases, pharmaceuticals, and environmental factors on hepatic oxidative metabolism activity in humans and animals in vivo, owing to its physicochemical, biochemical, and pharmacokinetic properties.

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

General description

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF2480V
BCCJ4268
BCCG5095
BCCF2841
BCCC1469

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Qureshi, M.
Mikrochimica Acta, 831-831 (1970)
Serum antipyrine concentrations determined by micellar electrokinetic capillary chromatography
Brunner LJ, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 622(1), 98-102 (1993)
Antipyrine, indocyanine green, and lorazepam determined in plasma by high-pressure liquid chromatography.
Awni WM and Bakker LJ
Clinical Chemistry, 35(10), 2124-2126 (1989)
Simultaneous determination of creatinine and uric acid in human plasma and urine by micellar electrokinetic chromatography
Miyake M, et al.
Journal of High Resolution Chromatography, 14(3), 181-185 (1991)
Automated high-performance liquid chromatographic determination of antipyrine and its main metabolites in plasma, saliva and urine, including 4, 4?-dihydroxy-antipyrine
Teunissen MWE, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 278(3), 367-378 (1983)
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