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A92775

Sigma-Aldrich

Arsenazo III

calcium-sensitive dye

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Synonym(s):
2,2′-(1,8-Dihydroxy-3,6-disulfonaphthylene-2,7-bisazo)bisbenzenearsonic acid, 2,7-Bis(2-arsonophenylazo)chromotropic acid
Linear Formula:
(HO)2C10H2(SO3H)2(N=NC6H4AsO3H2)2
CAS Number:
1668-00-4
Molecular Weight:
776.37
Beilstein:
5717957
EC Number:
216-788-6
MDL number:
MFCD00036695
PubChem Substance ID:
24891431
NACRES:
NA.47

Quality Level

200

form

powder

technique(s)

titration: suitable

impurities

<0.1% Ca

color

brown to very dark brown, and Black

mp

>320 °C (lit.)

solubility

ammonium hydroxide: 1 mg/mL

λmax

530 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(\N=N\c2ccccc2[As](O)(O)=O)c(cc3cc(c(\N=N\c4ccccc4[As](O)(O)=O)c(O)c13)S(O)(=O)=O)S(O)(=O)=O

InChI

1S/C22H18As2N4O14S2/c29-21-18-11(9-16(43(37,38)39)19(21)27-25-14-7-3-1-5-12(14)23(31,32)33)10-17(44(40,41)42)20(22(18)30)28-26-15-8-4-2-6-13(15)24(34,35)36/h1-10,29-30H,(H2,31,32,33)(H2,34,35,36)(H,37,38,39)(H,40,41,42)/b27-25+,28-26+

InChI key

UQHVTNUJRKELCE-NBHCHVEOSA-N

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Application

Arsenazo III has been used as a color forming reagent to determine uranium concentration.

Biochem/physiol Actions

Arsenazo III is a metallochromic dye. Arsenazo III is used in the determination of calcium in biological samples. It is used to evaluate calcium transport in permeabilized cells. It is also used for the detection of rare earth metals (polyvalent metal ions). Arsenazo III is used for the spectrophotometric determination of uranium and thorium.

Pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

Signal Word

Danger

Hazard Statements

H301 + H331 - H410

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Analytica Chimica Acta, 68, 73-73 (1974)
R Kuroda et al.
Talanta, 37(6), 619-624 (1990-06-01)
A derivative spectrophotometric method has been developed for the simultaneous determination of microgram quantities of uranium and thorium with Arsenazo III in hydrochloric acid medium. The second-derivative absorbances of the uranium and thorium Arsenazo III complexes at 679.5 and 684.4
J W Janssen et al.
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies, 29(3), 197-201 (1991-03-01)
The widely used calcium dye reagent, o-cresolphthalein complexone, was replaced by the less well known metallochromic indicator Arsenazo III in a selective multitest analyser. Calibration stability increased from 12 hours to more than 48 hours. With the Arsenazo III reagent
B R Morgan et al.
Clinical chemistry, 39(8), 1608-1612 (1993-08-01)
We describe an analytical procedure for determining serum calcium, using the ligand Arsenazo III in an aqueous alkaline medium. The choice of pH for the proposed technique differs from current procedures, which are for the most part carried out in
L J Dong et al.
Fresenius' journal of analytical chemistry, 370(8), 1009-1014 (2001-10-05)
The determination of proteins with arsenazo-DBN and Al3+ by Rayleigh light-scattering (RLS) is described. The weak RLS of arsenazo-DBN and BSA can be enhanced greatly by addition of Al3+ in the pH range 5.3-7.0; this resulted in two enhanced RLS
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