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Merck
CN

B2926

Brucella Agar

with Hemin and Vitamin K1, NutriSelect® Plus, powder, for Brucella spp., pack of 500  g, suitable for microbiology

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Plus
Ph:
7.0±0.2 (25 °C)
Form:
powder
Suitability:
Brucella spp., anaerobic bacteria, nonselective for Bacteroides spp., nonselective for Clostridium spp.
Application(s):
clinical testing
food and beverages
veterinary, microbiology
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sterility

non-sterile

form

powder

Quality Level

shelf life

limited shelf life, expiry date on the label

composition

with Hemin and Vitamin K1

packaging

pack of 500  g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

pH

7.0±0.2 (25 °C)

application(s)

clinical testing
food and beverages
veterinary, microbiology

suitability

Brucella spp., anaerobic bacteria, nonselective for Bacteroides spp., nonselective for Clostridium spp.

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Application

Used for the isolation and subculture of anaerobes.

Preparation Note

Suspend 43.1 g of Brucella Agar with Hemin and Vitamin K1 in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add sterile 5% v/v defibrinated sheep blood.

Other Notes

Components (g/L):
Casein Enzymic Hydrolysate 10.00
Peptic Digest of Animal Tissue 10.00
Yeast Extract 2.00
Dextrose 1.00
Sodium Chloride 5.00
Sodium Bisulphite 0.10
Hemin 0.10
Vitamin K1 0.01
Agar 15.00
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

Regulatory Information

动植物来源培养基
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Sutter, V.L, et al.
Wadsworth Anaerobic Bacteriology Manual (1985)
W M Weinstein et al.
Infection and immunity, 10(6), 1250-1255 (1974-12-01)
An animal model has been developed to study the evolution of intra-abdominal abscesses. Gelatin capsules containing pooled colonic contents and barium sulfate were prepared in an anaerobic chamber and implanted into the pelvic region of Wistar rats. The natural course
A B Onderdonk et al.
Infection and immunity, 10(6), 1256-1259 (1974-12-01)
An animal model simulating intra-abdominal sepsis was produced by implanting large bowel contents into the pelvic region of rats. Bacteriological analysis of infected sites showed quantitative differences according to the stage of disease. During the initial, often lethal, peritonitis stage

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