B3426
Bile salts mixture
suitable for microbiology
Synonym(s):
Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture
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About This Item
NACRES:
NA.74
UNSPSC Code:
41106212
sterility
non-sterile
Quality Segment
form
powder
packaging
pkg of 100 g
loss
≤6% loss on drying
color
yellow to brownish-yellow
pH
7±0.5
solubility
distilled water: freely soluble
application(s)
microbiology
storage temp.
10-30°C
General description
Bile salts mixture is a combination of an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Bile salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic, and lithocholic acids. Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that inhibits Gram-positive bacteria and induced germination. It also inhibits the outgrowth of the germinated spores but does not interfere with the growth of Enterobacteriaceae.
Application
Bile salts mixture is recommended in diverse media like MacConkey Agar, SS Agar, and Violet Red Bile Agar. It increases the selectivity of the media to Enterobacteriaceae by inhibiting Gram-positive bacteria. It is used as a selective agent in culture media for the selective growth of intestinal bacteria like E. coli, other fecal coliforms, and fecal Streptococci. But it is also used as a selective agent in media for the detection of Yersinia enterocolitica, Aeromonas hydrophila, and Vibrio species.
Preparation Note
Extracted from purified, fresh bile.
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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