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B9431

Biotin-4-Fluorescein

Name: Biotin-4-Fluorescein
Brand: SIAL

≥95% purity (HPLC), powder

Synonym(s):

5-(6)-[2-(biotinoyl)aminoethylaminocarbonyl] fluorescein, B4F, Biotin (5-fluorescein) conjugate

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About This Item

Empirical Formula (Hill Notation):

C₃₃H₃₂N₄O₈S

Molecular Weight:

644.69

MDL number:

MFCD09264223

UNSPSC Code:

12171500

eCl@ss:

34058011

PubChem Substance ID:

329773319

NACRES:

NA.47

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Properties

Product Name

Biotin-4-Fluorescein, ≥95.0% (HPLC)

Quality Level

100, 200

assay

≥95.0% (HPLC)

form

powder

mol wt

644.71

technique(s)

titration: suitable

color

light orange to very dark orange, light yellow to very dark yellow

ε (extinction coefficient)

≥70 at 491-497 nm in phosphate buffer at 0.05 M

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56

InChI

1S/C33H32N4O8S/c38-18-7-10-25-23(14-18)29(21-9-6-19(39)15-26(21)45-25)20-8-5-17(13-22(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30

InChI key

UROXVNHIYQXTRA-LFERIPGTSA-N

Description

Application

Biotin-4-Fluorescein has been used for the labeling of avidin to study the changes in protein binding capability after carbon negative-ion implantation into avidin. It has been used to study the binding affinity of streptavidin towards biotin.

Useful for quantitating biotin binding sites. This reagent binds to avidin more quickly than conventional fluorescein biotin.

Biochem/physiol Actions

Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenching, which is used for measuring the concentration of (strept)avidin.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Rapid estimation of avidin and streptavidin by fluorescence quenching or fluorescence polarization.

Kada G, et al.

Biochimica et Biophysica Acta, 1427, 44-48 (1999)

Coupled myosin VI motors facilitate unidirectional movement on an F-actin network.

Sivaraj Sivaramakrishnan et al.

The Journal of cell biology, 187(1), 53-60 (2009-09-30)

Unconventional myosins interact with the dense cortical actin network during processes such as membrane trafficking, cell migration, and mechanotransduction. Our understanding of unconventional myosin function is derived largely from assays that examine the interaction of a single myosin with a

Cell-Adhesion Patterning by using Carbon Negative-Ion Implantation into Albumin and Avidin Layers on Polystyrene.

Sommani P, et al.

Transactions of the Materials Research Society of Japan, 36.3, 289-292 (2011)

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Global Trade Item Number

SKU	GTIN
B9431-5MG	04061833443798