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Merck
CN

C1144

Chlorazol Black

Powder

Synonym(s):

4-Amino-3-[[4′-[(2,4-diaminophenyl)azo][1,1′-biphenyl]-4-yl]azo]-5-hydroxy-6-(phenylazo)-2,7-naphthalenedisulfonic acid disodium salt, Chlorazole Black E, Direct Black 38

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About This Item

Empirical Formula (Hill Notation):
C34H25N9Na2O7S2
CAS Number:
1937-37-7
Molecular Weight:
781.73
NACRES:
NA.47
PubChem Substance ID:
24892361
UNSPSC Code:
12171500
EC Number:
217-710-3
MDL number:
MFCD00066363
Colour Index Number:
30235

Key Documents

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Product Name

Chlorazol Black,

InChI key

XRPLBRIHZGVJIC-UVHCWRHYSA-L

InChI

1S/C34H27N9O7S2.2Na/c35-22-10-15-27(26(36)18-22)41-38-24-11-6-19(7-12-24)20-8-13-25(14-9-20)40-42-32-28(51(45,46)47)16-21-17-29(52(48,49)50)33(34(44)30(21)31(32)37)43-39-23-4-2-1-3-5-23;;/h1-18,44H,35-37H2,(H,45,46,47)(H,48,49,50);;/q;2*+1/p-2/b41-38+,42-40+,43-39+;;

SMILES string

[Na+].[Na+].Nc1ccc(\N=N\c2ccc(cc2)-c3ccc(cc3)\N=N\c4c(N)c5c(O)c(\N=N\c6ccccc6)c(cc5cc4S([O-])(=O)=O)S([O-])(=O)=O)c(N)c1

form

powder

composition

Dye content, ≥45% (based on Nitrogen)

color

dark brown to black

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Chlorazol Black has been used to monitor the infection of mycorrhizal Allium porrum in plant roots. It has also been used for the staining of copepods.

General description

Chlorazol Black has strong affinity for cellulose and chitin. In plant tissues, it helps to differentiate between cellulose and lignin. It also helps in distinguishing fungal bodies from artefacts due to chitin staining. It is also used as an auxiliary stain for chromosomes.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Carc. 1B - Eye Irrit. 2 - Repr. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS2080
SHBM4400
MKCD3320
SLBR5011V
SLBN7918V

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Mustafa Işik et al.
Journal of hazardous materials, 114(1-3), 29-39 (2004-10-30)
An Upflow Anaerobic Sludge Blanket Reactor/Continuous Stirred Tank Reactor was used sequentially to decolorize and mineralize C.I. Direct Black 38 azo dye (3200 mg/L) in a synthetic wastewater containing glucose as co-substrate. At the steady state conditions color was effectively
Cell-wall-bound peroxidase activity in roots of mycorrhizal Allium porrum.
Spanu P and Bonfante-Fasolo P
The New phytologist, 109, 119-124 (1988)
Gabriela Carvalho Collazzo et al.
Journal of environmental management, 98, 107-111 (2012-01-20)
The N-doped TiO(2) photocatalyst was prepared by calcination of a hydrolysis product composed of titanium (IV) isopropoxide with ammonia as the precipitator. X-ray diffraction, surface area, XPS and UV-vis spectra analyses showed a nanosized anatase structure and the appearance of
B Beije
Mutation research, 187(4), 227-234 (1987-04-01)
The genotoxic activity of the benzidine-derived azo dye, Direct Black 38 (DB38), was studied in vivo, using two different genetic end-points: unscheduled DNA synthesis in liver (UDS) and bone marrow micronucleus (MN). Exposure times were 12, 24 or 36 h.
Notes on Microscopical Technique for Zoologists null
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