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C1484

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin

Name: 7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin
Brand: SIAL

≥95% purity (HPLC), solid

Synonym(s):

1H-Pyrrole-2,5-dione, 1-(4-(7-(diethylamino)-4-methyl-2-oxo-2H-1-benzopyran-3-yl)phenyl)-, CPM

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About This Item

Empirical Formula (Hill Notation):

C₂₄H₂₂N₂O₄

CAS Number:

76877-33-3

Molecular Weight:

402.44

UNSPSC Code:

12171500

PubChem Substance ID:

24892413

NACRES:

NA.47

MDL number:

MFCD00077334

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Product Name

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin, ≥95% (HPLC), solid

Quality Level

100

assay

≥95% (HPLC)

form

solid

technique(s)

titration: suitable

color

yellow

solubility

DMSO: soluble, methanol: soluble

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CCN(CC)c1ccc2C(C)=C(C(=O)Oc2c1)c3ccc(cc3)N4C(=O)C=CC4=O

InChI

1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3

InChI key

YGIABALXNBVHBX-UHFFFAOYSA-N

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Related Categories

Clinical & Diagnostics

Hematology Stains

Histology Stains

Tissue Diagnostics

General description

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM), is a highly fluorescent, sulfhydryl (-SH) group containing coumarin derivative. It has excitation an emission wavelengths of 355 and 460 nm, respectively.

Application

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin has been used:

as a nontoxic substitute for conjugation with antibody(93)
to aid fluorescent detection of Coenzyme A (CoA-SH) in human N-myristoyltransferases assay (94)
in thermostability shift assay of recombinant protein(95)

Biochem/physiol Actions

Used to monitor release of thiols, quantitate thiol in microplate reactions, and to distinguish proliferating cancer cells by nucleolar protein staining.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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An essential role for the extracellular domain of the Na,K-ATPase beta-subunit in cation occlusion.

S Lutsenko et al.

Biochemistry, 32(26), 6737-6743 (1993-07-06)

The role of the Na,K-ATPase beta-subunit in stabilization of ion-binding sites has been investigated. Treatment of the purified renal Na,K-ATPase with 0.25 M DTT at 40 degrees C for 1 h resulted in 50% loss of Rb occlusion, which correlates

Somatosensory assessment and conditioned pain modulation in temporomandibular disorders pain patients.

Simple Futarmal Kothari et al.

Pain, 156(12), 2545-2555 (2015-08-27)

The pathophysiology and underlying pain mechanisms of temporomandibular disorders (TMD) are poorly understood. The aims were to assess somatosensory function at the temporomandibular joints (TMJs) and to examine whether conditioned pain modulation (CPM) differs between TMD pain patients (n =

A coupled fluorescent assay for histone methyltransferases.

Evys Collazo et al.

Analytical biochemistry, 342(1), 86-92 (2005-06-17)

Histone methyltransferases (HMTs) catalyze the S-adenosylmethionine (AdoMet)-dependent methylation of lysines and arginines in the nucleosomal core histones H3 and H4 and the linker histone H1b. Methylation of these residues regulates either transcriptional activation or silencing, depending on the residue modified

Mechanism of interactions of alpha-naphthoflavone with cytochrome P450 3A4 explored with an engineered enzyme bearing a fluorescent probe.

Tamara N Tsalkova et al.

Biochemistry, 46(1), 106-119 (2007-01-03)

Design of a partially cysteine-depleted C98S/C239S/C377S/C468A cytochrome P450 3A4 mutant designated CYP3A4(C58,C64) allowed site-directed incorporation of thiol-reactive fluorescent probes into alpha-helix A. The site of modification was identified as Cys-64 with the help of CYP3A4(C58) and CYP3A4(C64), each bearing only

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Global Trade Item Number

SKU	GTIN
C1484-25MG	04061833467626

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