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Merck
CN

C2734

5-Carboxytetramethylrhodamine

≥95% purity (HPLC), solid

Synonym(s):

5-TAMRA

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About This Item

Empirical Formula (Hill Notation):
C25H22N2O5
CAS Number:
Molecular Weight:
430.45
UNSPSC Code:
12171500
NACRES:
NA.47
MDL number:
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Product Name

5-Carboxytetramethylrhodamine,

assay

≥95% (HPLC)

Quality Segment

form

solid

solubility

water: slightly soluble

ε (extinction coefficient)

≥85000-97000 at 539-545 nm in methanol

fluorescence

λex ~543 nm; λem ~570 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

N(C)(C)c1cc2[o+]c3c(c(c2cc1)c4c(cc(cc4)C(=O)[O-])C(=O)O)ccc(c3)N(C)C

InChI

1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)

InChI key

YMZMTOFQCVHHFB-UHFFFAOYSA-N

General description

An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.

Application

5-Carboxytetramethylrhodamine has been used in fluorophore characterization.

Biochem/physiol Actions

5-Carboxytetramethylrhodamine can be covalently attached to the DNA and is thereby used in studies involving branched and duplex DNA/RNA structures. It is mainly used for single-molecule studies. 5-Carboxytetramethylrhodamine is also used for the labelling of oligosaccharide for laser-induced fluorescence detection.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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