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C4916

5-Carboxyfluorescein diacetate

~95% purity (HPLC), powder

Synonym(s):

5-CFDA, 5-Carboxy-di-O-acetylfluorescein

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About This Item

Empirical Formula (Hill Notation):
C25H16O9
CAS Number:
79955-27-4
Molecular Weight:
460.39
NACRES:
NA.47
PubChem Substance ID:
24892738
UNSPSC Code:
12171500
MDL number:
MFCD00036872
Beilstein/REAXYS Number:
68265
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Product Name

5-Carboxyfluorescein diacetate, ~95% (HPLC)

assay

~95% (HPLC)

Quality Level

200

form

powder

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(O)=O)c1

InChI

1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)

InChI key

WPUZGNPQMIWOHE-UHFFFAOYSA-N

Application

5-Carboxyfluorescein diacetate has been used for the staining of bacteria to study bacterial adhesion to mucin.

Biochem/physiol Actions

5-Carboxyfluorescein diacetate is required for the staining of cells to differentiate between live and dead cells. It helps in monitoring integrity and intactness of cell membrane. 5-Carboxyfluorescein diacetate is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product carboxyfluorescein. In viable cells, the cellular membrane is impermeable to the fluorescent molecule.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
0000258271
0000258272
0000236611
0000258272
0000258271

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Global Trade Item Number

SKUGTIN
C4916-100MG04061832341989
C4916-25MG04061832341996