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Merck
CN

C4916

5-Carboxyfluorescein diacetate

~95% purity (HPLC), powder

Synonym(s):

5-CFDA, 5-Carboxy-di-O-acetylfluorescein

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About This Item

Empirical Formula (Hill Notation):
C25H16O9
CAS Number:
79955-27-4
Molecular Weight:
460.39
NACRES:
NA.47
PubChem Substance ID:
24892738
UNSPSC Code:
12171500
MDL number:
MFCD00036872
Beilstein/REAXYS Number:
68265

Key Documents

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Product Name

5-Carboxyfluorescein diacetate, ~95% (HPLC)

InChI

1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(O)=O)c1

InChI key

WPUZGNPQMIWOHE-UHFFFAOYSA-N

assay

~95% (HPLC)

form

powder

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

Quality Level

200

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Application

5-Carboxyfluorescein diacetate has been used for the staining of bacteria to study bacterial adhesion to mucin.

Biochem/physiol Actions

5-Carboxyfluorescein diacetate is required for the staining of cells to differentiate between live and dead cells. It helps in monitoring integrity and intactness of cell membrane. 5-Carboxyfluorescein diacetate is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product carboxyfluorescein. In viable cells, the cellular membrane is impermeable to the fluorescent molecule.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
0000258271
0000258272
0000236611
0000258272
0000258271

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Hong Wei Ouyang et al.
Cell transplantation, 13(6), 649-657 (2005-01-15)
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Andreas W Sonesson et al.
Colloids and surfaces. B, Biointerfaces, 61(2), 208-215 (2007-09-25)
The adsorption and activity of Thermomyces lanuginosus lipase (TLL) was measured with dual polarization interferometry (DPI) and confocal microscopy at a hydrophilic and hydrophobic surface. In the adsorption isotherms, it was evident that TLL both had higher affinity for the
Maillard-type glycoconjugates from dairy proteins inhibit adhesion of Escherichia coli to mucin.
Food Chemistry, 129, 1435-1443 (2011)
Arend W Overeem et al.
Journal of hepatology, 71(2), 344-356 (2019-04-10)
Hepatocyte polarity is essential for the development of bile canaliculi and for safely transporting bile and waste products from the liver. Functional studies of autologous mutated proteins in the context of the polarized hepatocyte have been challenging because of the
Isabel Comino et al.
Food & nutrition research, 60, 30324-30324 (2016-02-09)
Oats provide important nutritional and pharmacological properties, although their safety in coeliac patients remains controversial. Previous studies have confirmed that the reactivity of the anti-33-mer monoclonal antibody with different oat varieties is proportional to the immune responses in terms of
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