C4916
5-Carboxyfluorescein diacetate
~95% purity (HPLC), powder
Synonym(s):
5-CFDA, 5-Carboxy-di-O-acetylfluorescein
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About This Item
Empirical Formula (Hill Notation):
C25H16O9
CAS Number:
Molecular Weight:
460.39
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
68265
Product Name
5-Carboxyfluorescein diacetate, ~95% (HPLC)
assay
~95% (HPLC)
Quality Segment
form
powder
solubility
chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(O)=O)c1
InChI
1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)
InChI key
WPUZGNPQMIWOHE-UHFFFAOYSA-N
Application
5-Carboxyfluorescein diacetate has been used for the staining of bacteria to study bacterial adhesion to mucin.
Biochem/physiol Actions
5-Carboxyfluorescein diacetate is required for the staining of cells to differentiate between live and dead cells. It helps in monitoring integrity and intactness of cell membrane. 5-Carboxyfluorescein diacetate is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product carboxyfluorescein. In viable cells, the cellular membrane is impermeable to the fluorescent molecule.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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