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Merck
CN

C9386

Casein Acid Hydrolysate for Biotechnology and Microbiology

for Biotechnology and Microbiology, ≥12.5% Total N, 8-12% Amino

Synonym(s):

Casein hydrolyzates, Hy-Case® SF, Peptone from casein, casein acid hydrolysate

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
41106212
EC Number:
265-363-1
NACRES:
NA.85
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Product Name

Hy-Case® SF, Casein acid hydrolysate, from bovine milk

biological source

bovine milk

Quality Level

form

powder

nitrogen analysis

8-12% amino, ≥12.5% total

loss

≤11% loss on drying

pH

5.4-7.4

application(s)

food and beverages
microbiology

storage temp.

10-30°C

suitability

Streptococcus spp., Streptomyces spp.

General description

Hy-Case® SF is a high-quality acid hydrolysis of casein. Casein is a rich source of amino nitrogen so on treatment with a strong acid, it gets hydrolysed, producing free amino acids and several short peptide fragments. Special processing keeps inorganic ions to a minimum, so Hy-Case® SF is virtually a high concentration of free amino acids with a minimum of salt. It is a refined grade hydrolysate with high clarity in solution and is food grade and is recommended where a mixture of highly soluble free amino acids is required. It is especially recommended when inorganic ion levels need to be kept at a minimum.

Application

Casein hydrolysate Hy-Case® SF was used in DMCY medium, during an experimental study conducted to investigate the role of ssgA gene product in promoting the sporulation and cell division in Streptomyces griseus. It may also be used in the YH medium in studies related to Halobacterium denitrificans sp. which is characterised as being carbohydrate-utilizing, denitrifying, extremely halophilic bacteria.
Recommended for use as a microbiological nutrient in laboratory media, fermentations, and tissue culture applications requiring a mixture of highly soluble free amino acids.

Preparation Note

Recommended concentration: 25 g/L

Other Notes

Similar to Hy-Case Amino, but contains a maximum of 2.0% sodium chloride

Legal Information

Hy-Case is a registered trademark of Kerry Group


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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物源性产品
低风险生物材料

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Articles

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.


G A Tomlinson et al.
International journal of systematic bacteriology, 36(1), 66-70 (1986-01-01)
Halobacterium denitrificans was one of several carbohydrate-utilizing, denitrifying, extremely halophilic bacteria isolated by anaerobic enrichment in the presence of nitrate. Anaerobic growth took place only when nitrate (or nitrite) was present and was accompanied by the production of dinitrogen.
S Kawamoto et al.
Microbiology (Reading, England), 143 ( Pt 4), 1077-1086 (1997-04-01)
The ssgA gene of Streptomyces griseus B2682, when present in high copy number, results in both suppression of sporulation and fragmented growth of mycelia. Western analysis with polyclonal antibodies against the gene product (SsgA) revealed a close correlation between SsgA



Global Trade Item Number

SKUGTIN
C9386-1KG04061833530597
C9386-500G04061833530603
C9386-5KG04061833662526