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D4784

4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride

Name: 4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride
Brand: SIAL

≥98% purity (HPLC), powder

Synonym(s):

1,2-Diamino-4,5-methylenedioxybenzene dihydrochloride, 1,3-Benzodioxole-5,6-diamine dihydrochloride, 5,6-Diamino-1,3-benzodioxole dihydrochloride, MDB

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About This Item

Empirical Formula (Hill Notation):

C₇H₈N₂O₂ · 2HCl

CAS Number:

81864-15-5

Molecular Weight:

225.07

NACRES:

NA.47

PubChem Substance ID:

24893897

UNSPSC Code:

12171500

MDL number:

MFCD00037497

Beilstein/REAXYS Number:

5641607

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Properties

Product Name

4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride, Fluorogenic reagent

Quality Level

100

assay

≥98% (HPLC)

form

powder

technique(s)

titration: suitable

color

white to orange

mp

247 °C

solubility

H₂O: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

Cl[H].Cl[H].Nc1cc2OCOc2cc1N

InChI

1S/C7H8N2O2.2ClH/c8-4-1-6-7(2-5(4)9)11-3-10-6;;/h1-2H,3,8-9H2;2*1H

InChI key

YXEJRYIEFFUUID-UHFFFAOYSA-N

Description

General description

4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride is a fluorescence tag for α keto acids. It is also required for the derivatization of sialic acids in presence of dilute sulfuric acid. The method allows determination of sialic acids.

Application

4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride has been used for the derivatization of sialic acids to analyse them by capillary liquid-chromatography-electrospray ionization/tandem mass spectrometry (CapLC-ESI-MS/MS).

Fluorogenic reagent for α-ketoacids.

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pictograms

GHS07

signalword

Warning

hcodes

H315,H319,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Improving the Chromatographic Separation of DMB-Labeled Sialic Acids for the Comparison of Biosimilars to Reference Materials

A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.

Determination of sialic acids in immune system cells (coelomocytes) of sea urchin, Paracentrotus lividus, using capillary LC-ESI-MS/MS.

Savaş İzzetoğlu et al.

Fish & shellfish immunology, 36(1), 181-186 (2013-11-13)

Coelomocytes are considered to be immune effectors of sea urchins. Coelomocytes are the freely circulating cells in the body fluid contained in echinoderm coelom and mediate the cellular defence responses to immune challenges by phagocytosis, encapsulation, cytotoxicity and the production