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Merck
CN

D9805

Fast Blue B Salt

Dye content ~95 %, Powder

Synonym(s):

o-Dianisidine bis(diazotized) zinc double salt, Azoic Diazo No. 48, DBB, Diazo Blue B, Naphthanil Diazo Blue B

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About This Item

Linear Formula:
C14H12N4O2Cl2 · ZnCl2
CAS Number:
14263-94-6
Molecular Weight:
475.47
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24894113
EC Number:
238-153-2
MDL number:
MFCD00075203
Colour Index Number:
37235
Beilstein/REAXYS Number:
1499067

Key Documents

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Product Name

Fast Blue B Salt, Dye content ~95 %

InChI

1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2

InChI key

GPPKNJIWDULNQH-UHFFFAOYSA-J

description

Technical grade

form

powder

composition

Dye content, ~95%

technique(s)

microbe id | staining: suitable

color

yellow to dark brown

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Quality Level

200

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Application

  • Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.
  • It has been used as a spraying agent in thin layer chromatography for the identification of separated components.
  • It has also been used for the determination of the lipase activity.

Biochem/physiol Actions

Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity.

pictograms

FlameHealth hazard
GHS02,GHS08

signalword

Danger

hcodes

H242,H350

Hazard Classifications

Carc. 1B - Self-react. C

Storage Class

5.2 - Organic peroxides and self-reacting hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Information

监管及禁止进口产品
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Certificates of Analysis (COA)

Lot/Batch Number
SHBT3017
MKCK4645
MKCG1594
MKCC1010
MKBZ2692V

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C N Kennett et al.
Journal of clinical periodontology, 24(6), 424-431 (1997-06-01)
Gingival crevicular fluid (GCF) was collected from chronic periodontitis patients using plastic micropipettes and coverslip smears stained with antibodies for leukocyte markers and Toluidine Blue for mast cells. The smears consisted of 70-80% granulocytes, 10-20% monocytes/macrophages, 5% mast cells and
Z Lojda
The Histochemical journal, 17(10), 1063-1089 (1985-10-01)
The histochemical demonstration of the activities of proteases, based in the majority of cases on the use of discriminating synthetic substrates (aminoacyl or peptidyl derivatives of 4-methoxy-2-naphthylamine [MNA]), has proved very valuable in pathology. Its importance is illustrated by three
A rapid TLC bioautographic method for the detection of acetylcholinesterase and butyrylcholinesterase inhibitors in plants.
Marston A, et al.
Phytochemical Analysis, 13, 51-51 (2002)
Overcoming the bell-shaped dose-response of cannabidiol by using Cannabis extract enriched in cannabidiol.
Gallily R, et al.
Pharmacology & Pharmacy, 6, 75-75 (2015)
Evaluation of acid phosphatase as a confirmation test for Clostridium perfringens isolated from water.
Sartory DP, et al.
Letters in Applied Microbiology, 42, 418-418 (2006)
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