Skip to Content
Merck
CN

Key Documents

View All Documentation

D9805

Fast Blue B Salt

Dye content ~95 %, Powder

Synonym(s):

o-Dianisidine bis(diazotized) zinc double salt, Azoic Diazo No. 48, DBB, Diazo Blue B, Naphthanil Diazo Blue B

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Linear Formula:
C14H12N4O2Cl2 · ZnCl2
CAS Number:
14263-94-6
Molecular Weight:
475.47
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24894113
EC Number:
238-153-2
MDL number:
MFCD00075203
Beilstein/REAXYS Number:
1499067
Colour Index Number:
37235
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Fast Blue B Salt, Dye content ~95 %

description

Technical grade

Quality Segment

200

form

powder

composition

Dye content, ~95%

technique(s)

microbe id | staining: suitable

color

yellow to dark brown

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2

InChI

1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4

InChI key

GPPKNJIWDULNQH-UHFFFAOYSA-J

Application

  • Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.
  • It has been used as a spraying agent in thin layer chromatography for the identification of separated components.
  • It has also been used for the determination of the lipase activity.

Biochem/physiol Actions

Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity.

pictograms

FlameHealth hazard
GHS02,GHS08

signalword

Danger

hcodes

H242,H350

Hazard Classifications

Carc. 1B - Self-react. C

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

supp_hazards

EUH044

Storage Class

4.1A - Other explosive hazardous materials

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library