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E1903

Ethidium homodimer I solution

≥95% purity (HPLC), liquid

Synonym(s):

EtDi, EthD-1, Phenanthridinium, 5,5′-[1,2-ethanediylbis(imino-3,1-propanediyl)]bis(3, 8-diamino-6-phenyl)-,dichloride,dihydrochloride

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About This Item

Empirical Formula (Hill Notation):
C46H50Cl4N8
Molecular Weight:
856.75
NACRES:
NA.47
PubChem Substance ID:
24894444
UNSPSC Code:
12171500
EC Number:
200-664-3
MDL number:
MFCD00210948
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Product Name

Ethidium homodimer I solution, ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC), 2 mM in DMSO

Quality Segment

100

assay

≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC)

form

liquid

concentration

2 mM in DMSO

technique(s)

titration: suitable

color

red

ε (extinction coefficient)

≥9000 at 490-496 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28

InChI

1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H

InChI key

ZKAJUKABUUJRLB-UHFFFAOYSA-N

Application

Ethidium homodimer I solution has been used to check membrane integrity in sperms.
Stains ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA structures.
Used to detect DNA in solution.

Biochem/physiol Actions

Ethidium homodimer is a red fluorescent dye. It cannot cross an intact plasma membrane, but can easily pass through a damaged plasma membrane. Ethidium homodimer is used to check membrane integrity.
One molecule of EthD-1 will non-selectively bind four base pairs in dsDNA.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

185.0 °F - closed cup

flash_point_c

85 °C - closed cup

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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