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E1903

Ethidium homodimer I solution

Name: Ethidium homodimer I solution
Brand: SIAL

≥95% purity (HPLC), liquid

Synonym(s):

EtDi, EthD-1, Phenanthridinium, 5,5′-[1,2-ethanediylbis(imino-3,1-propanediyl)]bis(3, 8-diamino-6-phenyl)-,dichloride,dihydrochloride

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About This Item

Empirical Formula (Hill Notation):

C₄₆H₅₀Cl₄N₈

Molecular Weight:

856.75

NACRES:

NA.47

PubChem Substance ID:

24894444

UNSPSC Code:

12171500

EC Number:

200-664-3

MDL number:

MFCD00210948

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Product Name

Ethidium homodimer I solution, ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC), 2 mM in DMSO

Quality Level

100

assay

≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC)

form

liquid

concentration

2 mM in DMSO

technique(s)

titration: suitable

color

red

ε (extinction coefficient)

≥9000 at 490-496 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28

InChI

1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H

InChI key

ZKAJUKABUUJRLB-UHFFFAOYSA-N

Related Categories

Clinical & Diagnostics

Hematology Stains

Histology Stains

Tissue Diagnostics

Application

Ethidium homodimer I solution has been used to check membrane integrity in sperms.

Stains ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA structures.

Used to detect DNA in solution.

Biochem/physiol Actions

Ethidium homodimer is a red fluorescent dye. It cannot cross an intact plasma membrane, but can easily pass through a damaged plasma membrane. Ethidium homodimer is used to check membrane integrity.

One molecule of EthD-1 will non-selectively bind four base pairs in dsDNA.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

185.0 °F - closed cup

flash_point_c

85 °C - closed cup

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Double bands in DNA gel electrophoresis caused by bis-intercalating dyes.

C Carlsson et al.

Nucleic acids research, 23(13), 2413-2420 (1995-07-11)

Many bis-intercalating dyes used for fluorescence detection of DNA in electrophoresis have been reported to give band-splitting and band-broadening, which results in poor resolution and a decreased detection sensitivity. We have studied the dimeric dye YOYO-1, and to some extent

Desiccation tolerance in bovine sperm: a study of the effect of intracellular sugars and the supplemental roles of an antioxidant and a chelator.

Sitaula R, et al.

Cryobiology, 58, 322-330 (2009)

Intercalative interactions of ethidium dyes with triplex structures.

E Tuite et al.

Bioorganic & medicinal chemistry, 3(6), 701-711 (1995-06-01)

The binding of phenanthridine dyes to triplex poly(dT)*poly(dA).poly(dT) and its precursor duplex poly(dA).poly(dT) is characterized using linear dichroism and circular dichroism spectroscopy, and thermal denaturation. The two monomeric dyes ethidium bromide and propidium iodide are shown to behave similarly to

Facile assembly of an affordable miniature multicolor fluorescence microscope made of 3D-printed parts enables detection of single cells.

Samuel B Tristan-Landin et al.

PloS one, 14(10), e0215114-e0215114 (2019-10-11)

Fluorescence microscopy is one of the workhorses of biomedical research and laboratory diagnosis; however, their cost, size, maintenance, and fragility has prevented their adoption in developing countries or low-resource settings. Although significant advances have decreased their size, cost and accessibility

Global Trade Item Number

SKU	GTIN
E1903-.5ML	04061838207067

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