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E2028

Ethidium bromide monoazide

Name: Ethidium bromide monoazide
Brand: SIAL

≥95% purity (HPLC), solid

Synonym(s):

3-Amino-8-azido-5-ethyl-6-phenylphenanthridinium bromide, Ethidium monoazide bromide

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About This Item

Empirical Formula (Hill Notation):

C₂₁H₁₈BrN₅

CAS Number:

58880-05-0

Molecular Weight:

420.31

UNSPSC Code:

12171500

PubChem Substance ID:

329799025

NACRES:

NA.47

MDL number:

MFCD01708677

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Properties

Product Name

Ethidium bromide monoazide, ≥95% (HPLC), solid

Quality Level

100

assay

≥95% (HPLC)

form

solid

technique(s)

titration: suitable

color

orange

solubility

DMF: soluble, ethanol: soluble

ε (extinction coefficient)

≥5200 at 459-465 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

[Br-].CC[n+]1c(-c2ccccc2)c3cc(ccc3c4ccc(N)cc14)N=[N+]=[N-]

InChI

1S/C21H17N5.BrH/c1-2-26-20-12-15(22)8-10-18(20)17-11-9-16(24-25-23)13-19(17)21(26)14-6-4-3-5-7-14;/h3-13,22H,2H2,1H3;1H

InChI key

GHUXAYLZEGLXDA-UHFFFAOYSA-N

Description

Biochem/physiol Actions

Photoactive stain covalently binds to nucleic acids in solution and in cells with damaged membranes.

Used to footprint drug binding sites on DNA, detect non-viable cells, identify ethidium binding sites on DNA and tRNA, and selectively inactivate gene expression.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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T cell epigenetic remodeling and accelerated epigenetic aging are linked to long-term immune alterations in childhood cancer survivors.

Sara Daniel et al.

Clinical epigenetics, 10(1), 138-138 (2018-11-08)

Cancer treatments have substantially improved childhood cancer survival but are accompanied by long-term complications, notably chronic inflammatory diseases. We hypothesize that cancer treatments could lead to long-term epigenetic changes in immune cells, resulting in increased prevalence of inflammatory diseases in

Methodologies for quantifying culturable, viable, and total Legionella pneumophila in indoor air.

C-W Chang et al.

Indoor air, 21(4), 291-299 (2011-01-05)

Legionella pneumophila, aerosolized from numerous indoor facilities (e.g., shower heads, hot tubs, spas), may cause Pontiac fever (PF) and lethal pneumonia named Legionnaires' disease (LD) in humans. Reliable methods on quantitative exposure assessment of this bioaerosol are essential for the

Escape from thymic deletion and anti-leukemic effects of T cells specific for hematopoietic cell-restricted antigen.

Ji-Min Ju et al.

Nature communications, 9(1), 225-225 (2018-01-18)

Whether hematopoietic cell-restricted distribution of antigens affects the degree of thymic negative selection has not been investigated in detail. Here, we show that T cells specific for hematopoietic cell-restricted antigens (HRA) are not completely deleted in the thymus, using the

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Global Trade Item Number

SKU	GTIN
E2028-5MG	04061833602430