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Merck
CN

E6003

Eosin Y disodium salt

Dye content ≥85%, Powder

Synonym(s):

2′,4′,5′,7′-Tetrabromofluorescein disodium salt, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, D&C;Red No. 22, Eosin yellowish

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About This Item

Empirical Formula (Hill Notation):
C20H6Br4Na2O5
CAS Number:
17372-87-1
Molecular Weight:
691.85
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24894349
EC Number:
241-409-6
MDL number:
MFCD00005040
Colour Index Number:
45380
Beilstein/REAXYS Number:
3586809

Key Documents

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Product Name

Eosin Y disodium salt, ≥85 % by HPLC

InChI key

SEACYXSIPDVVMV-UHFFFAOYSA-L

InChI

1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

SMILES string

[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24

form

powder

technique(s)

titration: suitable

color

brown

pH

6.55 (29 °C, 84 °F)

mp

255 — 270 °C (491 - 518 °F at 977.9 hPa)

solubility

H2O: 1 mg/mL

density

1.018 g/cm3

Quality Level

200

composition

, ≥85% HPLC

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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Application

Eosin Y disodium salt has been used for determining sperm viability. The swollen and stained spermatozoa were considered dead. In corneal epithelium, it has been used in the procedure of immunohistological staining.

Biochem/physiol Actions

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317,H319

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

376.9 °F - Pensky-Martens closed cup

flash_point_c

191.6 °C - Pensky-Martens closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT3805
SHBT3807
SHBS5617
WXBF3330V
WXBF4578V

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Quality of sperm obtained by penile vibratory stimulation and percutaneous vasal sperm aspiration in men with spinal cord injury.
Qiu Y, et al.
Journal of Andrology, 33, 1036-1036 (2012)
A comparison of lyophilized amniotic membrane with cryopreserved amniotic membrane for the reconstruction of rabbit corneal epithelium.
Ahn J, et al.
Biotechnology and Bioprocess Engineering: BBE, 10, 262-269 (2005)
Bancroft JD
Theory and Practice of Histological Techniques (2008)
Histophatologic Techniques null
Gangqi Wang et al.
Cells, 9(4) (2020-04-03)
We recently reported that loss of hyaluronan (HA) from the endothelial glycocalyx leads to loss of vessel stability in specific microcirculatory vascular beds. Here we hypothesized that such derangements in the glycocalyx may also impair the adaptive response to vascular
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