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Merck
CN

F7258

Fast Green FCF

certified by the BSC

Synonym(s):

Food green 3

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About This Item

Linear Formula:
C37H34N2O10S3Na2
CAS Number:
2353-45-9
Molecular Weight:
808.85
EC Number:
219-091-5
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24894936
MDL number:
MFCD00013053
Colour Index Number:
42053
Beilstein/REAXYS Number:
5718212

Key Documents

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Product Name

Fast Green FCF, certified by the BSC

InChI key

RZSYLLSAWYUBPE-UHFFFAOYSA-L

InChI

1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O

agency

certified by the BSC

form

powder

composition

dye content, ≥90%

mp

290 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Related Categories

Application

Certified for the safranin-fast green FCF stain for plant cells; as a substitute for aniline blue in Gomori′s one-step trichrome procedure.
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Fast Green FCF has been used:
  • along with safranin for the staining of nodule sections
  • in the collagen deposit staining procedure
  • for the staining of protein and starch
  • as a dye to detect and inject DNA solution in chicken embryo

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

hcodes

H335,H341

Hazard Classifications

Muta. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

352.4 °F - closed cup

flash_point_c

178 °C - closed cup

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT0602
SHBS0596
SHBR3287
SHBQ7677
SHBP1255

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Combination of in ovo electroporation and time-lapse imaging to study migrational events in chicken embryos.
Masyuk M et al.
Developmental Dynamics, 243, 690-690 (2014)
Micro-morphology of common bean (Phaseolus vulgaris L.) nodules undergoing senescence.
Fernandez-Luque?o F et al.
Acta Physiologiae Plantarum / Polish Academy of Sciences, Committee of Plant Physiology Genetics and Breeding, 30, 545-552 (2008)
Involvement of the Protein Network in thein vitroDegradation of Starch from Spaghetti and Lasagne: a Microscopic and Enzymic Study.
Fardet A et al.
Journal of Cereal Science, 27, 133-145 (1998)
Yohann Rautureau et al.
Circulation, 138(16), 1677-1692 (2018-04-21)
Pharmacogenomic studies have shown that ADCY9 genotype determines the effects of the CETP (cholesteryl ester transfer protein) inhibitor dalcetrapib on cardiovascular events and atherosclerosis imaging. The underlying mechanisms responsible for the interactions between ADCY9 and CETP activity have not yet
Nan Hu et al.
Arthritis research & therapy, 21(1), 167-167 (2019-07-10)
While bone marrow-derived mesenchymal stem cells (BMSC) are established sources for stem cell-based cartilage repair therapy, articular cartilage-derived mesenchymal stem cells from osteoarthritis patients (OA-MSC) are new and potentially attractive candidates. We compared OA-MSC and BMSC in chondrogenic potentials, gene
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