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F9252

Sigma-Aldrich

Folin & Ciocalteu′s phenol reagent

suitable for determination of total protein by Lowry method, 2 N

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MDL number:
NACRES:
NA.47

form

liquid

Quality Level

concentration

2 N

color

clear yellow

pH

<0.5 (20 °C)

density

1.240 g/cm3 at 20 °C

suitability

suitable for determination of total protein by Lowry method

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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This Item
47641TP0200M1942
concentration

2 N

concentration

2 M (with respect to acid)

concentration

-

concentration

-

color

clear yellow

color

-

color

-

color

-

pH

<0.5 (20 °C)

pH

-

pH

-

pH

-

density

1.240 g/cm3 at 20 °C

density

1.27 g/mL at 20 °C

density

-

density

-

suitability

suitable for determination of total protein by Lowry method

suitability

-

suitability

-

suitability

-

General description

Folin & Ciocalteu′s phenol reagent is most commonly used in the Lowry method for determining protein concentration. It has also been used for the quantification of total phenolics. In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution
stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750nm) or shoulder (660nm) are used to quantitate protein concentrations between 1-100 mg/ml while absorbance at 550nm is used to quantitate higher protein concentrations.
In the absence of copper, color intensity is determined primarily by the tyrosine and tryptophan content of the protein, and to a lesser extent by cysteine and histidine. Copper(II) has no effect on color formation by tyrosine, tryptophan, or histidine, but reduces color formation due to cysteine.
Many modifications of the original assay procedure have been published, including methods for enhancing the color development, for determining the content of insoluble proteins, and for automating the procedure. Compounds including many buffers, chelating agents, detergents, and cyclic organic compounds can interfere with the Lowry protein assay.
Folin & Ciocalteu′s phenol reagent can also be used as a spray reagent in chromatographic procedures.

Application

Folin & Ciocalteu′s phenol reagent has been used for the estimation of total phenolic content in samples.

Other Notes

Folin & Ciocalteu′s phenol reagent does not contain phenol. Rather, the reagent will react with phenols and non-phenolic reducing substances to form chromogens that can be detected spectrophotometrically.

Linkage

This product is also available as part of a kit.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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  1. What concentrations of proteins does Product F9252, Folin & Ciocalteu's phenol reagent, detect?

    Generally, Folin & Ciocalteu's phenol reagent is used to determine protein concentrations between 1 to 100 μg/mL. However, the reagent exhibits a linear response to protein concentration up to 1000 μg/mL when used according to the instructions for kit Product No. TP0200.

  2. Can Product F9252, Folin & Ciocalteu's phenol reagent, be used to detect any protein?

    If the protein has an abnormally low tyrosine content, this method would not be suitable.

  3. What should Product F9252, Folin & Ciocalteu's phenol reagent, look like?

    The Folin & Ciocalteu's phenol reagent should be a clear yellow solution.  If the solution turns green, it may not be suitable and it should not be used.

  4. Does Product F9252, Folin & Ciocalteu's phenol reagent, contain phenol?

    The Folin & Ciocalteu's phenol reagent does not contain phenol but reacts with phenol and non-phenolic reducing substances to form chromogens that can be detected spectrophotometrically.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent.
Ainsworth EA and Gillespie KM
Nature Protocols, 2, 875-875 (2007)
David L Jones et al.
PloS one, 9(3), e90882-e90882 (2014-03-19)
Monitoring the properties of dissolved organic carbon (DOC) in soil water is frequently used to evaluate changes in soil quality and to explain shifts in freshwater ecosystem functioning. Using >700 individual soils (0-15 cm) collected from a 209,331 km(2) area
Picheswara Rao Polu et al.
BMC complementary and alternative medicine, 17(1), 457-457 (2017-09-13)
Tinospora cordifolia (Guduchi or Amrita) is an important drug of Ayurvedic System of Medicine and found mention in various classical texts for the treatment of diseases such as jaundice, fever, diabetes, cancer and skin disease etc. In view of its
H J Fryer et al.
Analytical biochemistry, 153(2), 262-266 (1986-03-01)
The method of protein determination reported by Lowry et al. (1951, J. Biol. Chem. 193, 265-275) has been adapted for use with 96-well microtiter plates and an automatic microplate spectrophotometer. The spectrophotometer has been interfaced with a computer which plots
Han-Shin Kim et al.
Scientific reports, 6, 25318-25318 (2016-05-05)
Biofilm formation on biotic or abiotic surfaces has unwanted consequences in medical, clinical, and industrial settings. Treatments with antibiotics or biocides are often ineffective in eradicating biofilms. Promising alternatives to conventional agents are biofilm-inhibiting compounds regulating biofilm development without toxicity

Protocols

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Plant-Based PCR Inhibitors During DNA Extraction and PCR Master Mix Performance

Evaluating the effects of PCR inhibitors and PCR enzyme selection using plant genomic DNA, GenElute™-E Single Spin Plant Extraction Kit, KOD-One™ PCR Master Mix and competitor high fidelity PCR master mixes.

Enzymatic Assay of Proteinase K (EC 3.4.21.64)

Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).

Related Content

Tools for Protein Quantitation

A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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