F9252
liquid
2 N
clear yellow
<0.5 (20 °C)
1.240 g/cm3 at 20 °C
suitable for determination of total protein by Lowry method
diagnostic assay manufacturing
hematology
histology
room temp
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This Item | 47641 | TP0200 | M1942 |
---|---|---|---|
2 N | 2 M (with respect to acid) | - | - |
clear yellow | - | - | - |
<0.5 (20 °C) | - | - | - |
1.240 g/cm3 at 20 °C | 1.27 g/mL at 20 °C | - | - |
suitable for determination of total protein by Lowry method | - | - | - |
Danger
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
8B - Non-combustible, corrosive hazardous materials
WGK 2
Not applicable
Not applicable
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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How to Find a Lot/Batch Number for COA
Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.
For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').
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Generally, Folin & Ciocalteu's phenol reagent is used to determine protein concentrations between 1 to 100 μg/mL. However, the reagent exhibits a linear response to protein concentration up to 1000 μg/mL when used according to the instructions for kit Product No. TP0200.
If the protein has an abnormally low tyrosine content, this method would not be suitable.
The Folin & Ciocalteu's phenol reagent should be a clear yellow solution. If the solution turns green, it may not be suitable and it should not be used.
The Folin & Ciocalteu's phenol reagent does not contain phenol but reacts with phenol and non-phenolic reducing substances to form chromogens that can be detected spectrophotometrically.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Ask a Scientist here.
To standardize a procedure for the determination of protein by modified Lowry.
Evaluating the effects of PCR inhibitors and PCR enzyme selection using plant genomic DNA, GenElute™-E Single Spin Plant Extraction Kit, KOD-One™ PCR Master Mix and competitor high fidelity PCR master mixes.
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).
A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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