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I2752

Sigma-Aldrich

L-Isoleucine

reagent grade, ≥98% (HPLC)

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Synonym(s):
(2S,3S)-2-Amino-3-methylpentanoic acid
Linear Formula:
C2H5CH(CH3)CH(NH2)CO2H
CAS Number:
73-32-5
Molecular Weight:
131.17
Beilstein:
1721792
EC Number:
200-798-2
MDL number:
MFCD00064222
eCl@ss:
32160406
PubChem Substance ID:
24896006
NACRES:
NA.26

grade

reagent grade

Quality Level

200

Assay

≥98% (HPLC)

form

powder

color

white

mp

288 °C (dec.) (lit.)

solubility

1 M NH4OH: 50 mg/mL

application(s)

detection

SMILES string

CC[C@H](C)[C@H](N)C(O)=O

InChI

1S/C6H13NO2/c1-3-4(2)5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t4-,5-/m0/s1

InChI key

AGPKZVBTJJNPAG-WHFBIAKZSA-N

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Application

L-Isoleucine has been used in capillary feeder (CAFE) assay in a study to determine the consumption of amino acids by Drosophila melanogaster. It has also been used as one of the components of an amino acid mixture, which is used as a supplement for synthetic complete (SC) media.

Biochem/physiol Actions

L-Isoleucine is an isomer of L-leucine and is an essential amino acid. It is synthesized from threonine and is a branched-chain hydrophobic amino acid.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Conversion of ammonia or urea into essential amino acids,L-leucine,L-valine, andL-isoleucine using artificial cells containing an immobilized multienzyme system and dextran-NAD+
Applied Biochemistry and Biotechnology, 26 (1990)
Mated Drosophila melanogaster females consume more amino acids during the dark phase
Shun U
PLoS ONE (2017)
Inhibitory effect of aroma on the bitterness of branched-chain amino acid solutions.
Mukai J
Chemical & Pharmaceutical Bulletin, 55(11) (2007)
Amino Acid Biosynthesis Is Regulated by Feedback Inhibition
Biochemistry (5th Edition) null
Large-scale Phenotypic Profiling of Gene Deletion Mutants in Candida glabrata
Fabian Istel
Bio-protocol (2015)
View All Related Papers

Chromatograms

application for HPLC

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