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Merck
CN

L1886

Light Green SF Yellowish

Dye content, ≥65%, certified by the Biological Stain Commission, powder

Synonym(s):

Acid Green 5

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About This Item

Linear Formula:
C37H34N2O9S3Na2
CAS Number:
5141-20-8
Molecular Weight:
792.85
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24896295
EC Number:
225-906-5
MDL number:
MFCD00012121
Beilstein/REAXYS Number:
5717828

Key Documents

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Product Name

Light Green SF Yellowish, certified by the BSC

InChI

1S/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)\C(=C3\C=CC(\C=C3)=[N+](/CC)Cc4cccc(c4)S([O-])(=O)=O)c5ccc(cc5)S([O-])(=O)=O

InChI key

DGOBMKYRQHEFGQ-UHFFFAOYSA-L

agency

certified by the BSC

form

powder

composition

Dye content, ≥65%

technique(s)

titration: suitable

color

red to dark purple

mp

288 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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General description

Light Green SF Yellowish or Acid Green 5 is a triarylmethane dye that exists as disodium salt. It has the absorption value of 629 nm.

Application

Certified for use as a cytological counterstain and as a substitute for aniline blue in Gomori′s one-step trichrome procedure.
Light Green SF Yellowish functions as an efficient histological stain for collagen. It is also used as colorant for foodstuff and beverages.
Light Green SF Yellowish has been used to determine surface structures of trypsin treated tissue sections and to stain buccal cells for slide scoring.

Biochem/physiol Actions

Light Green SF Yellowish functions as an efficient histological stain for collagen. It is also used as colorant for foodstuff and beverages.

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS6661
SHBS3497
0000391071
0000391071
SHBR5685

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Atomic force microscope investigation of the boundary-lubricant layer in articular cartilage.
Chan SM
Osteoarthritis and Cartilage, 18, 956-956 (2010)
Decoloration treatment of a hazardous triarylmethane dye, Light Green SF (Yellowish) by waste material adsorbents
Mittal A, et al.
Journal of Colloid and Interface Science, 342(2), 518-527 (2010)
Buccal micronucleus cytome biomarkers may be associated with Alzheimer's disease.
Thomas P
Mutagenesis, 22, 371-371 (2007)
The buccal cytome and micronucleus frequency is substantially altered in Down's syndrome and normal ageing compared to young healthy controls
Thomas P, et al
Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 638, 37-47 (2008)
Atomic force microscope investigation of the boundary-lubricant layer in articular cartilage
Chan SMT, et al.
Osteoarthritis and Cartilage, 18(7), 956-963 (2010)
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