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L5382

Sigma-Aldrich

Light Green SF Yellowish

powder or crystals

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Synonym(s):
Acid Green 5
Linear Formula:
C37H34N2O9S3Na2
CAS Number:
5141-20-8
Molecular Weight:
792.85
Colour Index Number:
42095
Beilstein:
5717828
EC Number:
225-906-5
MDL number:
MFCD00012121
PubChem Substance ID:
24896400

form

powder or crystals

Quality Level

100

color

dark red to purple

mp

288 °C (dec.) (lit.)

solubility

water: 10 mg/mL, clear

εmax

>600 at 631-634 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)\C(=C3\C=CC(\C=C3)=[N+](/CC)Cc4cccc(c4)S([O-])(=O)=O)c5ccc(cc5)S([O-])(=O)=O

InChI

1S/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2

InChI key

DGOBMKYRQHEFGQ-UHFFFAOYSA-L

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General description

Light green SF yellowish (LGSFY) is a disodium salt of an acid triphenylmethane dye. It is also known as Light Green, Lissamine Green SF, Food Green 2, Green No. 205, Acid Green, Acid Brilliant Green 5, and Acid Brilliant Green SF. LGSFY is an important component of Papanicolaou stains in addition to eosin Y and bismark brown Y. Light green SF yellowish is a histological stain for collagen and a permitted colorant for foodstuffs and beverages.

Application

Light green SF is generally used as a cytoplasmic counterstain with nuclear stains like safranine and Feulgen stain. In histopatology, it is used for collagen fiber staining in Masson′s trichrome, Papanicolaou cytological polychrome stains, and Twort stain for microorganisms in tissue sections. It is a constituent of polychrome staining method used for differentiating secretory cells of the pituitary gland. Light green SF also stains the cellulose cell walls of plant cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Modification of apolipoprotein(a) lysine binding site reduces atherosclerosis in transgenic mice.
Boonmark NW
The Journal of Clinical Investigation, 100, 558-558 (1997)
Osteogenic differentiation as a result of BMP-2 plasmid DNA based gene therapy in vitro and in vivo.
Wegman F
European Cells & Materials, 21, 230-230 (2011)
Gurr E
Synthetic Dyes in Biology, Medicine And Chemistry (2012)
J S Drijver et al.
Acta cytologica, 27(6), 693-698 (1983-11-01)
The role of phosphotungstic acid as the main pH-controlling agent in Papanicolaou's EA stain was analyzed, including its effect on the competition between eosin Y and light green, the principal components of EA solutions, and this acid was compared with
Frank Schuettauf et al.
Investigative ophthalmology & visual science, 47(8), 3573-3578 (2006-08-01)
To investigate the effect of intravitreal injections of new vital dyes on the retina, the retinal pigment epithelium (RPE) and the choroid in an in vivo rat model. Rats were injected intravitreally with four dyes: light-green SF yellowish (LGSF), copper(II)phthalocyanine-tetrasulfonic
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