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Merck
CN

L5382

Light Green SF Yellowish

crystalline

Synonym(s):

Acid Green 5

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About This Item

Linear Formula:
C37H34N2O9S3Na2
CAS Number:
Molecular Weight:
792.85
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
225-906-5
MDL number:
Beilstein/REAXYS Number:
5717828
Colour Index Number:
42095
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Product Name

Light Green SF Yellowish, powder or crystals

form

powder or crystals

Quality Segment

technique(s)

titration: suitable

color

dark red to purple

mp

288 °C (dec.) (lit.)

solubility

water: 10 mg/mL, clear

εmax

>600 at 631-634 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)\C(=C3\C=CC(\C=C3)=[N+](/CC)Cc4cccc(c4)S([O-])(=O)=O)c5ccc(cc5)S([O-])(=O)=O

InChI

1S/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2

InChI key

DGOBMKYRQHEFGQ-UHFFFAOYSA-L

General description

Light green SF yellowish (LGSFY) is a disodium salt of an acid triphenylmethane dye. It is also known as Light Green, Lissamine Green SF, Food Green 2, Green No. 205, Acid Green, Acid Brilliant Green 5, and Acid Brilliant Green SF. LGSFY is an important component of Papanicolaou stains in addition to eosin Y and bismark brown Y. Light green SF yellowish is a histological stain for collagen and a permitted colorant for foodstuffs and beverages.

Application

Light green SF is generally used as a cytoplasmic counterstain with nuclear stains like safranine and Feulgen stain. In histopatology, it is used for collagen fiber staining in Masson′s trichrome, Papanicolaou cytological polychrome stains, and Twort stain for microorganisms in tissue sections. It is a constituent of polychrome staining method used for differentiating secretory cells of the pituitary gland. Light green SF also stains the cellulose cell walls of plant cells.


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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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