M0178
Middlebrook 7H9 Broth Base
suitable for microbiology, NutriSelect® Plus, Recommended for use in isolation and cultivation of Mycobacterium species
Quality Level
sterility
non-sterile
product line
BioChemika
form
powder
shelf life
limited shelf life, expiry date on the label
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbe id | susceptibility testing: suitable
microbiological culture: suitable
final pH
6.6±0.2 (25 °C)
application(s)
clinical testing
food and beverages
life science and biopharma
veterinary
microbiology
storage temp.
2-25°C
suitability
mycobacteria
Related Categories
General description
Middlebrook 7H9 Broth Base contains inorganic compounds that supply the elements potassium, sulfur, magnesium, and phosphorus which are required for appropriate growth of Mycobacteria. This medium with Middlebrook ADC Growth Supplement is recommended for ideal growth of Mycobacterium species.
Middlebrook ADC Growth Supplement (Product No. M0553) contains bovine albumin, dextrose, and catalase. Dextrose supplies energy, while albumin protects Mycobacteria from toxic agents and catalase splits toxic radicals.
Mycobacteria grow most rapidly in broth media and hence primary isolation of bacterial species can be carried out in Middlebrook 7H9 Broth Base.
Application
Components
Ammonium sulfate, 0.50
Disodium phosphate, 2.50
Monopotassium phosphate, 1.00
Sodium citrate, 0.10
Magnesium sulfate, 0.05
Calcium chloride, 0.0005
Zinc sulfate, 0.001
Copper sulfate, 0.001
Ferric ammonium citrate, 0.04
L-Glutamic acid, 0.50
Pyridoxine, 0.001
Biotin, 0.0005
Preparation Note
Footnote
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
related product
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Articles
The diseases produced by species of the genus Mycobacterium are important causes of morbidity and mortality in the world. The identification of mycobacteria to the species level is important because of the clinical significance; some species are pathogenic while others are not.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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