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Merck
CN

M0553

Middlebrook ADC Growth Supplement

Enrichment supplement recommended for the cultivation of Mycobacteria

Synonym(s):

Middlebrook Albumin Dextrose Catalase Supplement

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171614
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Quality Segment

sterility

sterile (Filtered and Aseptic Handled)

form

liquid

shelf life

limited shelf life, expiry date on the label

application(s)

clinical testing
environmental
food and beverages
life science and biopharma
microbiology
veterinary

storage temp.

2-8°C

suitability

mycobacteria

General description

Middlebrook ADC growth supplement is used to isolate a wide variety of Mycobacteria including M. tuberculosis. It contains Bovine albumin, dextrose, and catalase. Bovine albumin neutralizes the toxic products. Dextrose is the fermentable sugar and catalase cleaves the toxic hydrogen peroxides which creates stress to the Mycobacteria cells thus, making it an ideal media for the cultivation of Mycobacterial species

Application

Specialized culture media supplement, designed to isolate and cultivate various Mycobacterial species.

Preparation Note

Warm up the refrigerated supplement to 45-50°C. Aseptically add the contents of the vial to 450 ml of sterile Middlebrook 7H9 Broth Base (M0178). Mix well and pour into sterile screw capped tubes.

Other Notes

Ingredients: (per vial)
Bovine albumin fraction V 2.5g
Dextrose 1.0g
Catalase 0.0015g
Distilled water 50 ml


Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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