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Merck
CN

M6880

Malachite Green oxalate salt

Powder, crystals or chunks

Synonym(s):

N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium oxalate, Basic Green 4

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About This Item

Empirical Formula (Hill Notation):
C23H25N2 · C2HO4 · 0.5C2H2O4
CAS Number:
Molecular Weight:
463.50
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
219-441-7
Beilstein/REAXYS Number:
3644550
Colour Index Number:
42000
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Product Name

Malachite Green oxalate salt, Technical grade

InChI key

REZFCYOVFCJTDG-UHFFFAOYSA-M

InChI

1S/C23H25N2.C2H2O4/c1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3-1(4)2(5)6/h5-17H,1-4H3;(H,3,4)(H,5,6)/q+1;/p-1

SMILES string

C[N+](C)=C1C=CC(C=C1)=C(C2=CC=C(N(C)C)C=C2)C3=CC=CC=C3.[O-]C(C(O)=O)=O

grade

technical grade

form

powder, crystals or chunks

technique(s)

microbe id | staining: suitable

color

metallic green

solubility

water: 1 mg/mL

λmax

616-620 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

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Application

Malachite Green oxalate salt has been used in biliary phospholipid analysis.
Malachite Green oxalate salt has been used:
  • in the preparation of malachite green-ammonium molybdate reagent, as a stop solution for starch synthase activity assay
  • to terminate the phosphatase and tensin homologue deleted on chromosome 10 (PTEN) inhibition assay using phosphatidylinositol (3,4,5) triphosphate (PI(3,4,5)P3) as substrate
  • to determine trypanothione synthetase activity to measure inorganic phosphate release from adenosine triphosphate (ATP) during catalysis

Biochem/physiol Actions

Malachite green is used in the treatment of fungal and parasitic infections in fish.

General description

The release of phosphate or pyrophosphate in an enzymatic reaction can be detected by malachite green and is measured spectrometrically at 660nm.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Andrea C Mesías et al.
Free radical biology & medicine, 130, 23-34 (2018-10-26)
Chagas cardiomyopathy, caused by Trypanosoma cruzi infection, continues to be a neglected illness, and has a major impact on global health. The parasite undergoes several stages of morphological and biochemical changes during its life cycle, and utilizes an elaborated antioxidant
Targeting PTEN using small molecule inhibitors
Mak LH and Woscholski R
Methods, 77, 63-68 (2015)
Combinatorial Chemistry: From Theory to Application (2006)
Trypanothione synthetase confers growth, survival advantage and resistance to anti-protozoal drugs in Trypanosoma cruzi
Mesias AC, et al.
Free Radical Biology & Medicine, 130, 23-34 (2019)
ABCG5 and ABCG8 require MDR2 for secretion of cholesterol into bile.
Langheim S
Journal of Lipid Research, 46, 1732-1732 (2005)

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