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N2125

Naphthol AS-BI phosphate

≥98% purity (HPLC), powder

Synonym(s):

7-Bromo-3-hydroxy-2-naphthoic-o-anisidide phosphate

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About This Item

Empirical Formula (Hill Notation):
C18H15BrNO6P
CAS Number:
1919-91-1
Molecular Weight:
452.19
NACRES:
NA.47
PubChem Substance ID:
24897552
UNSPSC Code:
12171500
EC Number:
217-645-0
MDL number:
MFCD00004058
Beilstein/REAXYS Number:
6668526
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Product Name

Naphthol AS-BI phosphate, Technical grade, ≥98% (HPLC)

Quality Segment

assay

≥98% (HPLC)

form

powder

technique(s)

microbe id | staining: suitable

color

white to light yellow

solubility

methanol: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP(O)(O)=O

InChI

1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)

InChI key

HUXIAXQSTATULQ-UHFFFAOYSA-N

General description

Naphthol AS-BI, also known as 7-Bromo-3-hydroxy-2-naphtho-o-anisidine is a substituted naphthol.
Substrate for the histochemical demonstration of acid and alkaline phosphatase.

Application

Naphthol AS-BI phosphate is suitable to use:
  • in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
  • as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
  • to perform alkaline phosphatase reaction
Substrate for the histochemical demonstration of acid and alkaline phosphatase. Substrate for the fluorometric assay of acid and alkaline phosphatases. Demonstration of acid phosphatase in paraffin embedded tissue. Cytofluorometric assay of acid phosphatase in single living cells.

Biochem/physiol Actions

Naphthol AS-BI can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Gloves

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