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Merck
CN

N6876

Nitrotetrazolium Blue chloride

≥90.0% (HPLC)

Synonym(s):

2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

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About This Item

Linear Formula:
C40H30N10O6 · 2Cl
CAS Number:
298-83-9
Molecular Weight:
817.64
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24897795
MDL number:
MFCD00012159
Beilstein/REAXYS Number:
4115923

Key Documents

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Product Name

Nitrotetrazolium Blue chloride, ≥90.0% (HPLC)

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

assay

≥90.0% (HPLC)

form

powder

impurities

≤10% solvent

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Quality Level

200

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Related Categories

Application

  • Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
  • It has been used as a substrate for western blot detection.
  • It has been used for the staining of histological tissue sections.

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.

General description

Substrate for dehydrogenases and other oxidases.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2 - STOT SE 3

target_organs

Eyes,Central nervous system, Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS9948
SHBS9109
SHBS3161
MKCN4040
SHBR6484

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Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump.
Gaxiola RA et al.
Proceedings of the National Academy of Sciences of the USA, 98, 11444-11444 (2001)
Methods in Enzymology, 6, 958-958 (1963)
Fluorescent in situ hybridization employing the conventional NBT/BCIP chromogenic stain.
Trinh le A et al.
Biotechniques, 42, 756-756 (2007)
Expression of the endothelial lipase gene in murine embryos and reproductive organs.
Lindegaard ML et al.
Journal of Lipid Research, 46, 439-439 (2005)
Measurement of reactive oxygen intermediate production in haemocytes of the penaeid shrimp, Penaeus ?annamei.
Knaap V et al.
Aquaculture (Amsterdam, Netherlands), 191 , 89-107 (2000)
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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

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