N7005
Neutral Red
Dye content ≥90 %, Powder
Synonym(s):
3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red
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About This Item
Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
Molecular Weight:
288.78
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
209-035-8
MDL number:
Beilstein/REAXYS Number:
3918740
Colour Index Number:
50040
Product Name
Neutral Red, Dye content ≥90 %
form
powder
Quality Level
composition
Dye content, ≥90%
technique(s)
titration: suitable
color
brown to black
pH
6.8-8.0, red to yellow
mp
290 °C (dec.) (lit.)
solubility
water: 10 mg/mL, dark red
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1
InChI
1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
InChI key
PGSADBUBUOPOJS-UHFFFAOYSA-N
General description
Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.
Application
Neutral red has multiple staining applications that include its use as a nuclear stain, counterstain in the Gram procedure, either alone, or as a component of the Twort stain (a fast green-neutral red mixture). Neutral red staining is also a viability assessment method used for the detection of viable yeast cells in dandruff samples and of mussel larvae. Neutral red is also recommended for use in viability assessment as it is selectively absorbed by lysosomes and other acidic organelles in cells. It is also used for supravital staining of living blood cells.
Useful as an indicator for preparing neutral red paper, and as a biological stain.
Biochem/physiol Actions
Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.
Analysis Note
Purified
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Cell Growth, Differentiation and Senescence: A Practical Approach (1999)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| N7005-25G | 04061832343754 |
| N7005-5G | 04061834117810 |
| N7005-1G | 04061826116999 |