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NIST2141

Urea

NIST® SRM® 2141

Synonym(s):

Carbamide, Carbonyldiamide

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About This Item

Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
UNSPSC Code:
41116107
NACRES:
NA.24
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
635724
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InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

SMILES string

NC(N)=O

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

grade

certified reference material

form

crystals

packaging

pkg of 2 g

manufacturer/tradename

NIST®

Quality Level

mp

132-135 °C (lit.)

density

1.335 g/mL at 25 °C (lit.)

application(s)

clinical testing

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General description

Application

Urea Standard reference material (SRM) is certified for calibrating and standardizing microchemical procedures aimed at determining nitrogen in organic matter. Additionally, it serves as a tool for standardizing techniques for nitrogen determination, when carbon and hydrogen are simultaneously analyzed.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Features and Benefits

Available with a certificate documentingnitrogen value determined through a modification of the AOAC micro-Kjeldahlmethod.

Other Notes

Example analytes are listed below as a reference. Please download a current certificate at nist.gov/SRM for current analytes and certified values.
Nitrogen (N)

See certificate for values and more details at nist.gov/SRM.

Legal Information

NIST is a registered trademark of National Institute of Standards and Technology
SRM is a registered trademark of National Institute of Standards and Technology

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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