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P0914

Sigma-Aldrich

Protein standard

Micro Standard, liquid

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Synonym(s):
Albumin from bovine serum, protein standard
CAS Number:
9048-46-8
MDL number:
MFCD00130384
NACRES:
NA.27

biological source

bovine

Quality Level

200

form

liquid

contains

0.05% sodium azide as preservative

packaging

ampule of 1 mL

technique(s)

ELISA: suitable

color

clear colorless

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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Application

Concentrated solution for all protein assays.
Protein standard has been used:

  • as a standard in the quantification of protein release by bicinchoninic acid assay
  • as a standard in the quantification of urea by Bradfordassay
  • as a molecular size marker for the quantification of protein by native polyacrylamide gel electrophoresis
Protein standard for all protein assays. The sealed ampules provide assurance that the concentration will be correct with every assay.

Packaging

Sealed ampules

Other Notes

1 mg bovine serum albumin/ml in 0.15 M NaCl

Suitability

This product is commonly used for protein determination by the Bicinchoninic Acid (BCA) method.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

含少量动物源组分生物产品

Certificates of Analysis (COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Do you have any information on the traceability to a recognized protein standard, such as NIST? Or is the concentration derived from accepted values of natural physical constants?

    The concentration of each lot of the protein standard, Product P0914, is determined by UV measurements. Specifically, given a known extinction coefficient (E) and a measured absorbance (A), and assuming a standard pathlength of 1 cm, the concentration (c) can be determined by Beer's Law (c = A/E). The assay result is derived from an accepted value of a natural physical constant. Specifically, the E1% of 6.67 at approximately 279 nm is a well-accepted value, originally published in J. Am. Chem. Soc., 78, 3656-3660 (1956).

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