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P1754

TWEEN® 80

viscous liquid

Synonym(s):

TWEEN® 80, POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80

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About This Item

CAS Number:
9005-65-6
UNSPSC Code:
12161900
NACRES:
NA.21
MDL number:
MFCD00082107
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Quality Level

200

biological source

Porcine (tallow), bovine (tallow)

description

non-ionic

form

viscous liquid

mol wt

micellar avg mol wt 79,000, average mol wt 1310

composition

Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

aggregation number

60

CMC

0.012 mM (20-25°C)

viscosity

400-620 mPa.s

transition temp

cloud point 65 °C

solubility

water: soluble, clear to slightly hazy, colorless to yellow

density

1.064 g/cm3

HLB

15

application(s)

advanced drug delivery
cell analysis

SMILES string

CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

InChI key

RGPBUVUVZKQNHD-MDZDMXLPSA-N

General description

Tween® 80 is a non-ionic detergent and surfactant, classified as an ester of sorbitan and oleic acid. It is a versatile compound commonly employed in various biological applications, including cell lysis, nuclei isolation, and cell fractionation. Additionally, Tween 80 finds use in stabilizing proteins and facilitating protein membrane studies. Its effectiveness stems from its membership in the polysorbate group, characterized by the combination of sorbitol and sorbitane oleic esters with polyethylene glycol chains. These structural features contribute to Tween 80′s emulsifying capabilities and a broad range of applications.

Application

TWEEN® 80 has been used in drug vehicle solution for specific injections in mice and rats. It has been used for the determination of esterase activity in bacterial cells.
Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.

Biochem/physiol Actions

TWEEN® 80 is a polysorbate surfactant which helps in stabilizing proteins and in protein membrane studies. It is regularly used for the extraction of membrane proteins and isolation of nuclei from cells.

Features and Benefits

  • Excellent wetting and dispersing agent
  • Highly versatile surfactant for your Cell Biology and Biochemical research

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

TWEEN is a registered trademark of Croda International PLC

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Storage Class

10 - Combustible liquids

flash_point_f

>482.0 °F - Cleveland open cup

flash_point_c

> 250 °C - Cleveland open cup

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF8583V
BCCN6549
BCCN4705
BCCM9515
BCCM7843

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Global Trade Item Number

SKUGTIN
P1754-25ML04061837602399
P1754-10ML-KC04061838088253
P1754-500ML04061837602405
P1754-1GA04061837602375
P1754-1L04061837602382