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Merck
CN

P4030

Phloxine B

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

2′,4′,5′,7′-Tetrabromo-4,5,6,7-tetrachlorofluorescein disodium salt, Acid Red 92, Cyanosine, D&C;Red No. 28, Eosin 10B

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About This Item

Empirical Formula (Hill Notation):
C20H2Br4Cl4Na2O5
CAS Number:
Molecular Weight:
829.63
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
242-355-6
MDL number:
Beilstein/REAXYS Number:
3887100
Colour Index Number:
45410
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Product Name

Phloxine B, Dye content ≥80 %, certified by the BSC

agency

certified by the BSC

Quality Level

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

dark red

solubility

H2O: 1 mg/mL

εmax

1150-1300 at 546-550 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1c(Br)cc2c(Oc3c(Br)c([O-])c(Br)cc3C24OC(=O)c5c(Cl)c(Cl)c(Cl)c(Cl)c45)c1Br

InChI

1S/C20H4Br4Cl4O5.2Na/c21-5-1-3-17(9(23)15(5)29)32-18-4(2-6(22)16(30)10(18)24)20(3)8-7(19(31)33-20)11(25)13(27)14(28)12(8)26;;/h1-2,29-30H;;/q;2*+1/p-2

InChI key

OOYIOIOOWUGAHD-UHFFFAOYSA-L

General description

Phloxine B is part of the xanthylium class of dyes. The stain is mostly used to determine cell ploidy and temperature sensitivity of Schizosaccharomyces pombe. Solid media is colored a vivid pink.

Application

Phloxine B has been used as a component of yeast extract peptone dextrose (YEPD) to grow Schizosaccharomyces pombe.
Certified for use in Mallory′s phloxine-methylene blue stain; Kreyberg′s method for keratin and mucus.


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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Basic methods for fission yeast
Susan L
Yeast (2006)
The Small Subunit Processome Is Required for Cell Cycle Progression at G1
Kara A. Bernstein and Susan J. Baserga
Molecular Biology of the Cell (2004)
Advances in Food and Nutrition Research, 61 (2010)



Global Trade Item Number

SKUGTIN
P4030-25G04061834373476