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P4030

Sigma-Aldrich

Phloxine B

Dye content ≥80 %, certified by the Biological Stain Commission

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Synonym(s):
2′,4′,5′,7′-Tetrabromo-4,5,6,7-tetrachlorofluorescein disodium salt, Acid Red 92, Cyanosine, D&C;Red No. 28, Eosin 10B
Empirical Formula (Hill Notation):
C20H2Br4Cl4Na2O5
CAS Number:
18472-87-2
Molecular Weight:
829.63
Colour Index Number:
45410
Beilstein:
3887100
EC Number:
242-355-6
MDL number:
MFCD00070627
PubChem Substance ID:
24898486
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

composition

Dye content, ≥80%

color

dark red

solubility

H2O: 1 mg/mL

εmax

1150-1300 at 546-550 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1c(Br)cc2c(Oc3c(Br)c([O-])c(Br)cc3C24OC(=O)c5c(Cl)c(Cl)c(Cl)c(Cl)c45)c1Br

InChI

1S/C20H4Br4Cl4O5.2Na/c21-5-1-3-17(9(23)15(5)29)32-18-4(2-6(22)16(30)10(18)24)20(3)8-7(19(31)33-20)11(25)13(27)14(28)12(8)26;;/h1-2,29-30H;;/q;2*+1/p-2

InChI key

OOYIOIOOWUGAHD-UHFFFAOYSA-L

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General description

Phloxine B is part of the xanthylium class of dyes. The stain is mostly used to determine cell ploidy and temperature sensitivity of Schizosaccharomyces pombe. Solid media is colored a vivid pink.

Application

Phloxine B has been used as a component of yeast extract peptone dextrose (YEPD) to grow Schizosaccharomyces pombe.

Suitability

Certified for use in Mallory′s phloxine-methylene blue stain; Kreyberg′s method for keratin and mucus.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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