P4864
Propidium iodide solution
solution (1.0 mg/ml in water)
Assay
≥94%
Quality Level
form
solution (1.0 mg/ml in water)
solubility
water: 1 mg/mL
εmax
5400-6400 at 491-495 nm in phosphate buffer
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
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Related Categories
General description
Application
- in staining DNA
- in the viability staining method to study islet viability
- to visualize F-actins and nuclei in mesenchymal stem cell sheets
related product
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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1000309185
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Does Propidium Iodide, Product P4864, stain viable or non-viable cells?
Propidium Iodide does not generally enter living cells, However it may be taken up by axonal terminals and retrogradely transported to neuronal cell bodies. Reference: Aschoff, A. and H. Hollander, Fluorescent compounds as retrograde tracers compared with horseradish peroxidase (HRP). I. A parametric study in the central visual system of the albino rat. J. Neurosci. Methods, 6(3), 179-197 (1982).
What are the excitation and emission maxima for Propidium Iodide, Product P4864?
Excitation maximum is at 493 nm in water. Emission maximum is at 636 nm in water
How is Propidium Iodide, Product P4864, used for Flow Cytometry?
Immediately before flow cytometric analysis, prepare a 0.5 mg/mL solution of propidium iodide by combining 50μL of 2X PBS with 50 μL of P4864. Add this solution to 1 mL of a cell suspension containing 10,000,000 cells. (Note: Cells exhibiting fluorescence above 630 nm should be excluded from further analysis.)
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Articles
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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