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Merck
CN

P6892

Pyronin B

Dye content ≥30 %, Powder

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About This Item

Empirical Formula (Hill Notation):
C42H54Cl8Fe2N4O2
CAS Number:
2150-48-3
Molecular Weight:
1042.22
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24898803
EC Number:
218-429-9
MDL number:
MFCD03701356
Beilstein/REAXYS Number:
3846969

Key Documents

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Product Name

Pyronin B, Dye content ≥30 %

InChI key

SUVPQRYDMCNTPV-UHFFFAOYSA-J

InChI

1S/C21H27N2O.4ClH.Fe/c1-5-22(6-2)18-11-9-16-13-17-10-12-19(23(7-3)8-4)15-21(17)24-20(16)14-18;;;;;/h9-15H,5-8H2,1-4H3;4*1H;/q+1;;;;;+3/p-4

SMILES string

[Cl-].Cl[Fe](Cl)Cl.CCN(CC)c1ccc2cc3ccc(cc3[o+]c2c1)N(CC)CC

form

powder

composition

Dye content, ≥30%

mp

174-176 °C (lit.)

solubility

water: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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General description

Pyronin B is a chromogenic reagent, and is optimal for myeloperoxidase staining, which is useful in cytomorphological diagnosis and also in leukaemia categorization.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBM6002
SHBM5340
MKCK1572
MKCB9138
MKCB2876V

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Quick staining technique for myeloperoxidase using potassium iodide and oxidized pyronine B.
Chen W X, et al.
Acta Histochemica, 116(1), 292-296 (2014)
Feng Gao et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 60(11), 2505-2509 (2004-08-06)
This is the first report on the determination of nucleic acids with Pyronine B (PB) sensitized by cetyltrimethylammonium bromide (CTMAB) with resonance light-scattering (RLS) technique. Under the experimental conditions (1 x 10(-5) mol l(-1) PB, 1 x 10(-5) mol l(-1)
Belén Reija et al.
The journal of physical chemistry. B, 109(4), 1364-1370 (2006-07-21)
The aim of this work is to analyze the role of specific interactions in host-guest association processes. The formation of inclusion complexes between pyronines Y and B and beta-cyclodextrin and the nature of the interactions involved have been studied using
C A Mannella et al.
Biochimica et biophysica acta, 981(2), 363-366 (1989-06-06)
Inhibition of mitochondrial respiration by hydrophobic fluorescent dyes (Rhodamine 6G, Safranine O, Pyronine B) is much less potentiated by digitonin-lysis of the outer membrane than that by polyamines or adriamycin. This situation may be explained by impermeability of the anion-selective
Nuclear dyes and cytoplasmic staining.
G Diaz et al.
Cytometry, 29(4), 375-376 (1998-01-24)
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