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P8341

Sigma-Aldrich

TWEEN® 20

Low-peroxide; Low-carbonyls

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Synonym(s):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
CAS Number:
9005-64-5
MDL number:
MFCD00165986
NACRES:
NA.21

Quality Level

200

description

non-ionic

form

viscous liquid

mol wt

~1228

impurities

≤0.5 μmol/g Peroxides
≤1.0 μmol/g Carbonyls
≤3.0% water

refractive index

n20/D 1.468 (lit.)

CMC

0.06 mM (20-25°C)

transition temp

cloud point 76 °C

density

1.095 g/mL at 25 °C (lit.)
1.1 g/cm3 at 25 °C (lit.)

HLB

16.7

storage temp.

−20°C

SMILES string

CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI key

HMFKFHLTUCJZJO-UHFFFAOYSA-N

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General description

Tween®20, is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

TWEEN 20 is widely used in several biochemical applications. It has been used:
  • As an emulsifying agent for the preparation of stable oil-in-water emulsions
  • In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
  • As a blocking agent for membrane based immunoassays at a typical concentration of 0.05%
  • For lysing mammalian cells at a concentration of 0.005 to 0.5%
Non-ionic detergent

Other Notes

Preservative Free

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

527.0 °F - Pensky-Martens closed cup

Flash Point(C)

275 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Martindale: The Extra Pharmacopoeia, 1030-1030 null
Detergents: an overview.
J M Neugebauer
Methods in enzymology, 182, 239-253 (1990-01-01)
The role of transforming growth factor beta isoforms in tendon-to-bone healing
Kim H M, et al.
Connective tissue research, 52(2), 87-98 (2011)
Polysorbates 20 and 80 used in the formulation of protein biotherapeutics: structure and degradation pathways.
Kerwin BA
Journal of Pharmaceutical Sciences, 97(8), 2924-2935 (2008)
M Hultdin et al.
Nucleic acids research, 26(16), 3651-3656 (1998-08-01)
Determination of telomere length is traditionally performed by Southern blotting and densitometry, giving a mean telomere restriction fragment (TRF) value for the total cell population studied. Fluorescence in situ hybridization (FISH) of telomere repeats has been used to calculate telomere
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